What are the best practice of waste disposal plant tissue culture media?

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How can rhizobial strains be evaluated without plant experiments?

Hi everyone, I'm looking for a method to evaluate the nodulation ability and effectiveness of different strains of rhizobia. Is there any method that avoids testing in plants? Are there any...

17 July 2024 7,249 0 View

Are the coordinates the center of the cell or a corner in GADM / worldclim maps in R?

I am currently using a map downloaded from https://gadm.org/maps/DEU/bayern.html and I would like to assign the value of a variable to each cell with R. I was also trying to get this information...

15 July 2024 8,844 1 View

Is it recommended to use DNA LoBind tubes for protein extraction - and how, if at all, does it affect retrieval of proteins?

Hi, in our group we are trying to optimize a protein extraction protocol, and I've noticed DNA LoBind tube and DNA LoBind tips are being used. I wanted to know if it is ok to use DNA LoBind for...

30 June 2024 7,751 2 View

Is there any equipment that replaces GPC/SEC in carrageenan characterization?

22 June 2024 2,361 3 View

What should I use to homogenize samples for measurements of Oxidative Stress?

I bought a lipid peroxidation kit from Sigma and I want to get kits to measure nitrates, superoxide dismutase, catalase, and glutathione. Each one of those kits comes with a different lysis...

07 May 2024 4,583 2 View

¿Qué autores hablan sobre teorías sociales para abordar los desafíos de los adolescentes en las redes sociales?

Con esta pregunta me gustaría indagar sobre aquellos autores que abordan las teorías sociales para analizar los desafíos de los adolescentes en las redes sociales para obtener una visión mas...

10 March 2024 3,813 3 View

¿Cómo pueden los Trabajadores sociales ayudar a las personas con discapacidad intelectual a explorar su identidad sexual?

Las personas con discapacidad se enfrentan a barreras emocionales como el estigma y la infravaloración social, provocando carencias en la autoestima y el bienestar social de estas. La...

10 March 2024 6,010 1 View

Conservación de esporas de hongos micorricicos arbusculares?

Buenos días necesito, aislar esporas HMA de distintos suelos para su identificación, pero no se cómo conservarlas en el tiempo para poder mandarlas a identificar. Yo sé aislarlas pero no sé cual...

19 February 2024 781 2 View

Do you think Kindness is important at Working Organizations?

Hello, I am conducting a survey on Kindness at your Workplace and I would love to hear from you. It is all anonymous and super fast to complete. Here is the link for it & kindest regards...

28 January 2024 2,313 2 View

Cómo puedo compartir un trabajo, ya que no me deja hacerlo?

Respuesta micorrícica a la aplicación de inóculos de hongos micorrícicos arbusculares nativos en simbiosis con Neltuma alba = Mycorrhizal response to the application of inoculums of arbuscular...

03 January 2024 8,606 2 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Microtome tissue sectioning - How to prevent chatter of paraffin tissue specimen sections?

Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...

06 August 2024 666 4 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Good Day, can I ask for your help on the questionnaire that I want to adopt?

I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates

03 August 2024 3,505 6 View

Can i quantify a nanoparticle encapsulated in cell culture medium by hplc?

Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...

03 August 2024 2,730 1 View

How to Transform my handy-wavy explanation of why group I/II metals oxides in aqueous solution are basic into a rigorous argument?

I heard an explanation about something being a better proton acceptor or lone pair donor but that doesn't make sense. I couldn't explain in in terms of acid-base theory. The hand-waving way I saw...

03 August 2024 9,918 0 View

What is the problem with these tissue culture plants?

All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.

01 August 2024 589 4 View