Mônica Bossardi Coelho

9 Questions 10 Answers 0 Followers

Questions related from Mônica Bossardi Coelho

In meristem regeration, aiming shoot development: What auxin is the best choice to replace the use of Indole-3-Propionic Acid (IPA)?

The original media recipe, contains IPA 0,5 mg/L.

29 April 2025 1,083 0 View

I'm looking for a simple and efficient protocol for micropropagation of petunia hybrida starting from nodal explants. Does someone have an indication?

I also am looking to avoid callus in my culture.

17 February 2023 8,657 3 View

What is the reason to use egg yolks in explant disinfection process in plant tissue culture?

One example: Egg yolks with sodium hypochlorite 20% and tween 20.

26 December 2022 1,412 1 View

Many plants have a milky sap (latex) when they are cutted. In micropropagation, how can we proceed with them?

How can we manipulate them without spreading this sticky liquid in our tools and media culture? As it is the first time I work with milky sap plants, I appreciate all the experienced advices.

01 December 2022 709 3 View

What are the best practice of waste disposal plant tissue culture media?

And for PGR solutions?

29 November 2022 7,305 2 View

To adjust the pH of plant tissue culture medium: What can be used to replace HCL without comprimising the experiment quality?

Thanks for your help.

05 November 2022 3,334 4 View

What can be used to adjust the pH of plant tissue culture medium?

Thanks for your help.

05 November 2022 7,797 4 View

What is the light intensity needed in plant tissue culture when it comes to slow growth storage?

SLOW GROWTH STORAGE for the mid-term conservation of a large number of species, including tropical and temperates. In this case, reducing the in vitro growth through the application low...

01 January 1970 4,511 20 View

An appropriate and cheap method for grinding plant tissues for ELISA technique.

I am looking for good alternatives aiming to avoid buying expensive mashing bags for extraction. What kind of non-binding container are recommeded?

01 January 1970 3,886 3 View