What are the best kits for the in vitro transcription process?

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Any research paper call for publication related to management or finance or economics?

It should be standard journal (Indexed)

01 March 2021 8,379 1 View

What are the new researchable area under Rice crop under Agronomy related to moisture stress and nanotechnology ?

I had already worked on the moisture stress and lot of works being done in rice regarding stress or drought. I want to know some very recent researchable areas in rice crop regarding moisture...

26 January 2021 5,518 7 View

What will the effect on the inverse of large sparse (symmetric) matrix when a single (or a few) entries in the original matrix are perturbed?

The mathematics behind the inverse of large sparse matrices is very interesting and widely used in several fields. Sometimes, It is required to find the inverse of these kinds of matrices....

07 November 2020 4,842 3 View

How to calculate tilt angle and rotation angle of a membrane protein using gromacs 2018?

I have performed MD simulation to a trans membrane protein using gromacs 2018. Can anyone provide me way to calculate the tilt angle of the protein relative to bilayer? Also how can I calculate...

16 July 2020 7,917 1 View

How to clean organic matter deposited on quartz furnace tube. I used ethanol it did not work for me. Anyone please hep me?

I burnt waste organic matter (bagasse powder) at 400 C. Some sticky like substance is deposited on quartz tube. I want to clean that. Please help me.

30 July 2019 10,010 3 View

In dissolution of semisolid dosage form using immersion cell, how can we identify whether the membrane is intact or broken without opening the cell?

while removing the retaining ring to check whether membrane is intact or not, a crack develops in the membrane. Is there any alternative way to check the membrane.

17 May 2019 489 2 View

How can we differentiate a grassroots innovation or a frugal innovation from a local innovation ? Are they similar in their mode of working ?

What are the underlying notions that can distinguish these innovation in the informal settings?

12 May 2019 2,870 7 View

How do I extract the most correct esitmation of ion saturation current from a langmuir probe data by using curve fitting in matlab?

(1)What are the most popular implemented theories for describing the ion saturation region? (2) My I-V curve does not have a distinct electron saturation region.How do i get the most accurate...

28 February 2019 2,100 9 View

How do I get positions of atoms in a crystal if I only know Hermann Mauguin or space group of the crystal?

I can see a lot of information for one specific space group on the 'international table of crystallography'. Say I know the space group of a crystal from XRD analysis, and I want to calculate...

12 February 2019 6,328 7 View

Entropy Generation in Conductive Airways?

The inhaled air moves distally down to lung airways in process of breathing. Convection plays dominant criteria in gas mixing in conductive airways and as it moves further below diffusion starts...

12 October 2018 9,459 3 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

How to define tie constraint between rigid body and another part in dynamic explicit model of Abaqus?

Hi dear researchers I connected a rigid body into the part of my structure by tie constraint and then I added displacement to rigid body, in static general step every thing is fine and these two...

28 February 2021 3,442 2 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

What is the best concentration of cDNA to be used for qPCR?

I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...

24 February 2021 8,618 3 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Budak Drop out

use Ambion® MEGAscript® RNAi Kit from Life technologies.

Nirmala Jagadish

Roche is good and working.

Bharat Bhusan Patnaik

Dear friends and peers, thanks for the valuable advice. I have been using Ambion MEGAscript RNAi kit for in vitro transcription that yields good results but after the purification process suddenly the dsRNA value plummets significantly downwards. I have trying yo purify by 5M ammonium acetate method and i have good results. In one case 8 micrograms/microlitre.