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Do you have any suggestion, please share here. Thank you.
10 January 2015 1,743 20 View
I've got problems during filling the submission form for protein sequences. After filling the form and pressed the submit button, it stated as "can't open file". How can I solve this? thanks.
16 December 2014 9,591 5 View
I eluted purified his-tag protein in 200 ul of elution buffer containing 500 mM imidazole. Then, I want to use it for SDS-PAGE to confirm the presence of specific his-tag protein band on gel. What...
05 August 2013 4,008 11 View
Any available software for this purpose?
30 July 2013 576 2 View
Which method is suitable for precipitating protein from yeast cell lysate? From my own search, I found that the method of precipitating the protein is not a single method because there are a few...
24 July 2013 1,366 5 View
What factors are affecting this condition? separating gel- 12% stacking gel- 4% Even my protein marker did not show any bands in separating gel after staining with coomassie blue and destaining...
17 July 2013 3,306 27 View
Is it possible to run protein samples on agarose gel only?My predicted size of desired proteins is around 30-60 kDa. How much the concentration of agarose should be used for separating protein...
13 July 2013 1,301 9 View
What is the suitable temperature for keeping yeast liquid culture after incubating at 30c, 250 rpm for overnight? The type of yeast is saccharomyces cerevisiae. I plan to keep it first for a few...
06 July 2013 9,350 11 View
Which type of E. coli can be used as an expression vector for expressing heterologous protein of interest from plant-based genes?
01 April 2013 7,130 3 View
Can anyone explain briefly the differences between ISSR and RAPD?
25 March 2013 7,428 9 View
Is it possible for isoprenoid genes (fragrance, aroma, scent) to be expressed in kluyveromyces genes? Hopefully, I can get positive feedback from scientific communities who have expertise in...
19 March 2013 8,141 3 View
