Budak Drop out

12 Questions 41 Answers 0 Followers

Questions related from Budak Drop out

Which software is free and easier to use for RSM and CCD?

Do you have any suggestion, please share here. Thank you.

10 January 2015 1,801 20 View

How can one use TSP-PRED properly for submitting protein sequences?

I've got problems during filling the submission form for protein sequences. After filling the form and pressed the submit button, it stated as "can't open file". How can I solve this? thanks.

16 December 2014 9,644 5 View

Can someone advise on purified his-tag protein in elution buffer containing 500 mM imidazole (His Spin Trap kit - GE Healthcare)?

I eluted purified his-tag protein in 200 ul of elution buffer containing 500 mM imidazole. Then, I want to use it for SDS-PAGE to confirm the presence of specific his-tag protein band on gel. What...

05 August 2013 4,085 11 View

How to trim adaptor / linker sequences from EST sequences before submitting to NCBI?

Any available software for this purpose?

30 July 2013 645 2 View

Does anyone have experience with protein precipitation from yeast cell lysate (Saccharomyces cerevisiae)?

Which method is suitable for precipitating protein from yeast cell lysate? From my own search, I found that the method of precipitating the protein is not a single method because there are a few...

24 July 2013 1,420 5 View

No migration of protein sample from stacking gel to separating gel

What factors are affecting this condition? separating gel- 12% stacking gel- 4% Even my protein marker did not show any bands in separating gel after staining with coomassie blue and destaining...

17 July 2013 3,367 27 View

Does anyone have experience with running protein samples on agarose gel?

Is it possible to run protein samples on agarose gel only?My predicted size of desired proteins is around 30-60 kDa. How much the concentration of agarose should be used for separating protein...

13 July 2013 1,370 9 View

Storage temperature for yeast liquid culture (saccharomyces cerevisiae)

What is the suitable temperature for keeping yeast liquid culture after incubating at 30c, 250 rpm for overnight? The type of yeast is saccharomyces cerevisiae. I plan to keep it first for a few...

06 July 2013 9,401 11 View

E. coli as an heterologous protein expression host?

Which type of E. coli can be used as an expression vector for expressing heterologous protein of interest from plant-based genes?

01 April 2013 7,171 3 View

ISSR and RAPD

Can anyone explain briefly the differences between ISSR and RAPD?

25 March 2013 7,486 9 View

K. lactis and S. cerevisiae: which is the best for expressing heterologous recombinant protein derived from isoprenoid and phenylpropanoid genes of vandaceous orchids?

Are both hosts good for expressing proteins from fragrance genes?

20 March 2013 873 0 View

Recombinant protein expression in kluyveromyces lactis.

Is it possible for isoprenoid genes (fragrance, aroma, scent) to be expressed in kluyveromyces genes? Hopefully, I can get positive feedback from scientific communities who have expertise in...

19 March 2013 8,192 3 View