Bharat Bhusan Patnaik

5 Questions 20 Answers 0 Followers

Questions related from Bharat Bhusan Patnaik

What would be the perfect negative control for studying the RNAi efficiency?

I am using buffer control as well as EGFP-dsRNA as negative control for my target gene knockdown studies. When I compare the RNAi efficiency for my target gene with buffer as control and...

16 August 2013 2,131 2 View

What are the best kits for the in vitro transcription process?

For RNAi based functional analysis, many kits are on the market and found productive. Sometimes in vitro transcription step is followed by a quick protocol as ammonium acetate precipitation. But...

20 April 2013 9,239 3 View

Cloned insert (TA Cloning vector) in reverse orientation

I have been conducting TA cloning of my gene of interest for its expression but mostly I get sequencing results of the clone inserted in reverse orientation rather than the correct orientation....

01 June 2012 9,077 6 View

Double digestion of TOPO pCR 2.1 TA Cloning vector with the gene of interest not giving insert band of expected size

My gene of interest (Partial N-terminal fragment) have been cloned in TOPO TA Cloning vector-Invitrogen. I want to express the gene in the expression pET vector and produce the recombinant...

08 May 2012 2,608 11 View

I have purified my bioactive proteins by RP-HPLC (linear gradient flow of acetonitrile with water) with two peak fractions at 280nm absorbance. I have...

HPLC purified peak fractions lyophilized and run on 12% SDS-PAGE- No band observed with coommassie staining. Please suggest.

03 February 2012 4,660 5 View