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Questions related from Bharat Bhusan Patnaik
I am using buffer control as well as EGFP-dsRNA as negative control for my target gene knockdown studies. When I compare the RNAi efficiency for my target gene with buffer as control and...
16 August 2013 2,094 2 View
For RNAi based functional analysis, many kits are on the market and found productive. Sometimes in vitro transcription step is followed by a quick protocol as ammonium acetate precipitation. But...
20 April 2013 9,198 3 View
I have been conducting TA cloning of my gene of interest for its expression but mostly I get sequencing results of the clone inserted in reverse orientation rather than the correct orientation....
01 June 2012 9,026 6 View
My gene of interest (Partial N-terminal fragment) have been cloned in TOPO TA Cloning vector-Invitrogen. I want to express the gene in the expression pET vector and produce the recombinant...
08 May 2012 2,564 11 View
HPLC purified peak fractions lyophilized and run on 12% SDS-PAGE- No band observed with coommassie staining. Please suggest.
03 February 2012 4,621 5 View