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Questions related from Bharat Bhusan Patnaik
Lectin transcripts have been implicated in the innate immune mechanisms of invertebrates. I have studied its function through gene-silencing mechanisms. I need to devise strategies to over-express...
02 July 2015 5,532 1 View
I am using buffer control as well as EGFP-dsRNA as negative control for my target gene knockdown studies. When I compare the RNAi efficiency for my target gene with buffer as control and...
16 August 2013 2,136 2 View
For RNAi based functional analysis, many kits are on the market and found productive. Sometimes in vitro transcription step is followed by a quick protocol as ammonium acetate precipitation. But...
20 April 2013 9,243 3 View
Please let me know the best starting material/strategy for in vitro transcription process out of the following. 1- Circular plasmid (insert in TA cloning vector) followed by PCR with dsDNA...
18 April 2013 3,730 7 View
I have been conducting TA cloning of my gene of interest for its expression but mostly I get sequencing results of the clone inserted in reverse orientation rather than the correct orientation....
01 June 2012 9,082 6 View
My gene of interest (Partial N-terminal fragment) have been cloned in TOPO TA Cloning vector-Invitrogen. I want to express the gene in the expression pET vector and produce the recombinant...
08 May 2012 2,615 11 View
HPLC purified peak fractions lyophilized and run on 12% SDS-PAGE- No band observed with coommassie staining. Please suggest.
03 February 2012 4,664 5 View
