27 February 2021 3 703 Report

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure where this is coming from. Was it PCR or is it something that is because of sequencing error? If this is because of PCR, what could be the reasons why it happened and how to avoid it? Thanks! (Please see the image attached to the question)

The entire sequence is correct. I also saw the mutation I wanted. Only problem is at 3' end just before stop codon.

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