Dear All,
we formulated these drugs nanoparticles. however, we are not familiar with biological activity, types of cell lines and diseases. so kindly suggest which are the cell lines we can use to study above indicated drugs.
thanks in advance
Dear Raj
Please see the link http://www.ncbi.nlm.nih.gov/pmc/articles/PMC350074/pdf/pnas00496-0420.pdf
We had used Mouse L cells, 3T3 and human foreskin fibroblast lines for similar studies
For analgesics (nabumetone, Ibuprofen and Ketoprofen), the easiest method is to induce inflammation in rat or mice paw using any suitable agent such as formalin. After administring NPs, measure reduction in the size of inflammEd paw (in millimeters) caused by your drug loaded NP, taking pure drug solution as control. In cell lines studies, you will need cultures of cells involved in inflammatory process and measure reduction or induction of inflammatory mediators (interleukins and interferons etc) which may be complex. Biocompatibility studies may be performed by evaluating cytotoxicity of NP without drug (Hela cells by MTT assay). Lesser cell killing will mean NP are more biocompatible.
- Badges
- Science topic
- Similar topics
- Biological Science
- Molecular Cell Biology
- Culture Cells
- Cell Line