Hello,
I am trying to find a cheaper replacement for the item below. I've found a few options but i'm confused why some say 154 aa or 147 aa. I understand that the aa stands for amino acids but will this make a difference for what we are trying to use it for. We need it to supplement our cells that we are reprogramming from fibroblast to iPSCs.
https://www.thermofisher.com/antibody/product/Human-FGF-basic-FGF-2-bFGF-aa-1-155-Recombinant-Protein/PHG0264
Hello Mirella Perez
The primary translation product for bFGF is composed of 155 amino acids without a traditional signal peptide. The mature form of secreted human bFGF is 146 amino acids long (nine amino acid residues at the N-terminus are likely proteolytically cleaved from the 155-aa precursor). The biological activities of 155 aa form and 146 aa form are similar when tested under the same conditions, indicating that the N terminal region of bFGF is not involved either in biological activity or in binding to FGF cell surface receptors. Although there is no difference in biological function for these two forms, it is recommended that the 155 aa form be used for ES cells and the 146 aa form for neural cell studies.
For more information on bFGF you may refer to the references given below.
Article Human basic fibroblast growth factor: nucleotide sequence an...
Article Human brain fibroblast growth factor. Isolation and partial ...
Best.
- Badges
- Science method