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Questions related from Mirella Perez
Would transfection of iPSCs work using this plasmid? https://www.addgene.org/132776/ We already tried it using electroporation and lipostem but saw no green fluorescence. Could it be possible...
30 January 2023 9,627 4 View
Can someone recommend a good solution and tube material to freeze aav2/9 in a -80C freezer? Is a cryoprotectant required? We currently froze our AAV product in DPBS(+Cacl2,+MgCl2) into a cryovial...
15 December 2022 9,025 1 View
https://www.addgene.org/112867/ How realistic would it be to amplify a 15Kb plasmid with no errors? We are currently trying to amplify a plasmid needed for transfection. The problem is that the...
08 December 2022 8,552 8 View
Hello, I am trying to find a cheaper replacement for the item below. I've found a few options but i'm confused why some say 154 aa or 147 aa. I understand that the aa stands for amino acids but...
22 June 2022 135 1 View
Hi we recently reprogrammed fibroblasts into iPSCs, the morphology is off but we are wondering why they are still staining with tra-1-60? We picked the colonies to see if the morphology would...
10 March 2022 7,998 5 View
This is our first time reprogramming iPSCs. We reprogrammed fibroblasts using episomal vectors. Attached is a picture of reprogrammed fibroblasts at 4x on Day 19 (15 days of N2B27 +FGF and 4 days...
07 March 2022 2,336 5 View
