There are a number of reports for and against isolating CSCs from established cancer cell lines. One of the best work from Prof. Max Wicha lab, where they isolated CSCs from 33 breast cancer cell lines is attached. However, a number of my colleagues in North America, particularly in pharmaceutical industry, prefer CSCs from primary tumors for generating reliable "Hit-Lead Molecules" for further preclinical testing in appropriate animal models? Some of the prestigious journals' reviewers quite often questions the authenticity of CSCs isolated from cell lines? On the other hand, for obvious reasons, getting primary tumors and isolating CSCs are laborious, time consuming and resource intensive? What are my best options for studying the role of breast CSCs in metastasis?

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