I am performing conjugations where I am adding oligonucleotides to proteins. For my confirmation steps, I am doing SDS-PAGE and staining the gel using Pierce Silver Stain to observe shifts in the proteins. Since my product is usually in low abundance, I do not want to use more to run a different gel. Also, I need something that is very sensitive, fast, and relatively cheap. Is there anyway I can also stain for DNA on these gels? Thanks!
Hello
Silver-staining would do it for you. It has a very high resolution as well.
Have you tried this approach: Article Stabilizing Labile DNA-Protein Complexes in Polyacrylamide Gels
Silver staining can detect upto 1ng of protein/DNA and is more sensitive as compared to any other staining method (10 times more than Coomassie stains) I think silver staining is best for tracing DNA and Protein.
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