Joseph Compton Madden

10 Questions 5 Answers 0 Followers

Questions related from Joseph Compton Madden

What are good methods for staining trace DNA and protein in the same gel?

I am performing conjugations where I am adding oligonucleotides to proteins. For my confirmation steps, I am doing SDS-PAGE and staining the gel using Pierce Silver Stain to observe shifts in the...

24 January 2020 9,527 3 View

What are reliable resources for studying targets of miRNAs?

We are analzying changes in miRNA expression as a result of virus infection using Nanostring. While I have a lot of interesting hits in my data, I do not know the best way to determine the...

23 October 2019 7,270 3 View

Anyone use Thunder-Link PLUS Oligo conjugation system for conjugating something other than antibodies?

We want to barcode some peptides and were wondering if the procudure for doing so was significantly different than for doing it with antibodies. Is there any special considerations for using proteins?

16 October 2019 7,928 0 View

How do I determine viral genome copy number using a standard curve?

I am trying to quantify the level of viral RNA in mouse brains following infection using reverse transcription quantitative PCR. I have made a standard curve for determining the quantity (in ng)...

25 April 2016 8,851 2 View

What is a reliable company for producing antibodies?

We want to make some custom antibodies against viral nonstructural proteins. We wish to only provide the primary amino acid sequences. We did so in the past, but the company we worked with does...

04 January 2015 529 6 View

How can I fix low miniprep yields from glycerol stocks with an transmembrane protein?

I have glycerol stocks of transformed competent cells containing p3xFLAG-CMV-10 with ampicillin resistance and my gene of interest. The gene is a mouse oligodenylate synthetase protein with a...

27 November 2013 3,059 3 View

How can I isolate small PCR products (700-400 bp) for sequencing?

I need to isolate these fragments from a PCR reaction and then sequence them to see if they contain a conserved sequence. I have predicted product sizes that I am looking for, and while being able...

29 August 2013 6,396 20 View

Is it possible to reverse the orientation of a gene in a plasmid?

I was doing some cloning, and everything was going swimmingly. I only used a single restriction enzyme for the insertion (I know, I know, so sue me). Anyway, the gene was inserted in the incorrect...

28 June 2013 7,394 8 View

Are qiaprep and qiaquik spin columns interchangable?

I have seen the answer from the manufacturer, but I was curious if anybody has had any luck using them interchangeably

03 June 2013 2,956 3 View

Looking for effective minimal dilution of dsRNA antibody for floating tissue sections

I'm trying to measure replication of flavivirus in mouse brains using the Hungarian dsRNA antibody. This is the second time I've done the experiment, and I feel that the dilution I ran before was...

27 February 2013 7,048 0 View