What are different positive controls in experiment measuring lipid ROS using BODIPY(581/591)?

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Can the size of an empty micelle be larger than the size of the micelle in which the ingredient is encapsulated?

The particle size distribution of the micelles was measured using a particle size analyzer. The dv 50 value of the empty micelles was about 2 um larger than the dv 50 of the encapsulated micelles....

19 July 2024 803 4 View

Hybrid functional with meta-GGA in VASP?

Long story short, the VASP manual delineates that the metal-GGA could be utilized for hybrid functional with appropriate Fock operator using AEXX tag. However, there are no explicit notifications...

07 July 2024 2,713 3 View

Will this Piggybac transposon vector work well?

As you can see below, I cloned piggybac transposon vector which have two independent promoters (CMV-A, B, C gene / EF1a core-PuroR), but it didin't work well. After Electroporation with...

05 May 2024 9,380 0 View

What does ₩ mean in tcad?

Hello, I am studying about TCAD and I'm trying to understand one script file(sdevice) which is written for something device. For that script, I can find easily "₩" in the script. Could you tell me...

02 April 2024 6,684 0 View

ROS does not decrease after treating Andrographolide on HaCaT although Andrographolide increases mRNA transcription of NRF2 mech?

Hi, I'm MS student in skku. I am currently working on regulation of the NRF2 mechanism by andrographolide in HaCat cell. Andrographolide increases NRF2 mRNA level and transcriptional activity in...

10 March 2024 4,450 0 View

How can i uniform the thickness on curved surfaces during the Dip molding process?

As shown in the photo, the curved mandrel must be immersed in liquid TPU through a dip molding process. However, the outside of the bent part will always be thinly coated. Can you tell me the...

01 February 2024 9,504 2 View

In Materials Studio, I can't select an atom or atoms by clicking and dragging. What should I do?

In Materials Studio, I can't select an atom or atoms by clicking and dragging. I can select only bonds in 3D model by clicking or dragging. What's the problem?

30 January 2024 1,259 1 View

Is it okay to use liquid argon as an inert gas in a glove box?

Is it okay to use liquid argon as an inert gas in a glove box? I'm currently using argon gas, and it needs frequent replacements, which is inconvenient. I'm wondering if using liquid argon might...

12 December 2023 9,159 2 View

How to change the physical properties for all plot in one time in sdevice,TCAD?

When I want to check all .tdr file about i.e. Impactionization, I used to change dopingconcentration to impactionization one by one. But it's too time consuming as I think. Please let me know how...

08 December 2023 3,500 1 View

Will there be a short circuit when aluminum metal(probably aluminium oxide) enters the cathode of the secondary battery?

Will there be a short circuit when aluminum metal(probably aluminium oxide) enters the cathode of the secondary battery? Simply structure of Al foil (current collector)-Cathode-Al Oxide -...

13 November 2023 1,237 0 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View