Up till now, there are two kinds of 2x Laemmli sample buffers:

Buffer 1) 65.8 mM Tris-HCl, pH 6.8, 2.1% SDS, 26.3% (w/v) glycerol, 0.01% bromophenol blue

Please refer to the original paper: Cleavage of structural proteins during the assembly of the head of bacteriophage T4

http://www.academia.edu/3313242/Cleavage_of_structural_proteins_during_the_assembly_of_the_head_of_bacteriophage_T4

And BioRad, Cat# 161-0737.

Buffer 2) 4% SDS, 20% glycerol, 10% 2-mercaptoethanol, 0.004% bromphenol blue and 0.125 M Tris HCl, pH approx. 6.8

Please refer to Sigma, Cat# S3401. And Cold Spring Harbor Protocols.

Buffer 1) is for optimal band resolution based on BioRad manual.

Does anybody know the advantage of buffer 2)?

Thanks.

http://www.bio-rad.com/en-mx/sku/161-0737-2x-laemmli-sample-buffer

http://www.sigmaaldrich.com/catalog/product/sigma/s3401?lang=en®ion=US

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