FACSAria bad for absolute cell counts?

I recently heard that the FACSAria is not a good instrument for the evaluation of absolute counts using beads because of the construction of the flow cell and dual velocity that exists within the...

01 February 2016 6,761 2 View

What do you think of this centrifugation protocol for preparing plasma samples for microparticle analysis by flow cytometry from peripheral blood?

Peripheral blood tubes are immediately inverted 10 times and centrifuged at 4,200 x g, 7 min, with brake. The supernatant plasma is transferred to a new tube and centrifuged at 13,000 x g, 5 min...

31 December 2014 4,798 4 View

What is the standard protocol to induce bovine tissue factor expression using LPS?

I'd like to trial an antibody targeting tissue factor expression on bovine monocytes and I'm looking for the standard LPS-treatment to use as a positive control. What's your experience?

02 March 2014 2,287 5 View

Can someone recommend a multicolour immune cell activation flow cytometry panel?

I'm looking to monitor immune cell activation (neutrophils, monocytes, T/B/NK-cells) using multicolour flow cytometry and was wondering if anyone can recommend already established panels?

01 February 2014 3,318 11 View

What's the best activation marker antibodies to use for pig platelets?

I'm planning to investigate the activation status of porcine (pig) platelets as a response to various stimuli. What antibodies would you recommend for flow cytometry?

01 February 2014 5,619 6 View

Where can I buy Mab IL-A 110 targeting bovine neutrophils?

I want to identify bovine neutrophils in blood samples based on a surface marker, and not just by forward/side scatter. A few articles mention Mab IL-A 110 as a neutrophil-specific marker, but the...

31 December 2013 3,324 1 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Why do the air flow results vary totally when adding scalar in CFD ?

Dear All, We are using Autodesk cfd to calculate the air flow in a single sided naturally ventilated room, where air flow depends only on buoyancy due to temperature difference between inside and...

25 February 2021 9,602 4 View