Wharton's jelly single cell suspension?

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FACSAria bad for absolute cell counts?

I recently heard that the FACSAria is not a good instrument for the evaluation of absolute counts using beads because of the construction of the flow cell and dual velocity that exists within the...

01 February 2016 6,882 2 View

What do you think of this centrifugation protocol for preparing plasma samples for microparticle analysis by flow cytometry from peripheral blood?

Peripheral blood tubes are immediately inverted 10 times and centrifuged at 4,200 x g, 7 min, with brake. The supernatant plasma is transferred to a new tube and centrifuged at 13,000 x g, 5 min...

31 December 2014 4,916 4 View

What is the standard protocol to induce bovine tissue factor expression using LPS?

I'd like to trial an antibody targeting tissue factor expression on bovine monocytes and I'm looking for the standard LPS-treatment to use as a positive control. What's your experience?

02 March 2014 2,396 5 View

Can someone recommend a multicolour immune cell activation flow cytometry panel?

I'm looking to monitor immune cell activation (neutrophils, monocytes, T/B/NK-cells) using multicolour flow cytometry and was wondering if anyone can recommend already established panels?

01 February 2014 3,413 11 View

What's the best activation marker antibodies to use for pig platelets?

I'm planning to investigate the activation status of porcine (pig) platelets as a response to various stimuli. What antibodies would you recommend for flow cytometry?

01 February 2014 5,721 6 View

Where can I buy Mab IL-A 110 targeting bovine neutrophils?

I want to identify bovine neutrophils in blood samples based on a surface marker, and not just by forward/side scatter. A few articles mention Mab IL-A 110 as a neutrophil-specific marker, but the...

31 December 2013 3,405 1 View

What are the emerging trends and opportunities for STEM education and careers in the online space?

10 August 2024 507 0 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Why do we equate male and female arousal?

Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)

05 August 2024 9,537 2 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

How can I get my Granzyme B flow cytometry stain to be consistent?

I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...

01 August 2024 7,677 2 View

Best mask design for metal deposition aiming build a Perovskite solar cell device?

What types of mask designs for metal deposition, to be used in a PVD system, are best suited for perovskite-based solar cells?

31 July 2024 4,835 3 View