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Questions related from Ina Laura Pieper
I recently heard that the FACSAria is not a good instrument for the evaluation of absolute counts using beads because of the construction of the flow cell and dual velocity that exists within the...
02 February 2016 6,858 2 View
Peripheral blood tubes are immediately inverted 10 times and centrifuged at 4,200 x g, 7 min, with brake. The supernatant plasma is transferred to a new tube and centrifuged at 13,000 x g, 5 min...
01 January 2015 4,889 4 View
I would like to prepare a single cell suspension of Wharton's jelly for flow cytometry. Any protocol recommendations?
06 June 2014 7,672 2 View
I'd like to trial an antibody targeting tissue factor expression on bovine monocytes and I'm looking for the standard LPS-treatment to use as a positive control. What's your experience?
03 March 2014 2,376 5 View
I'm looking to monitor immune cell activation (neutrophils, monocytes, T/B/NK-cells) using multicolour flow cytometry and was wondering if anyone can recommend already established panels?
02 February 2014 3,384 11 View
I'm planning to investigate the activation status of porcine (pig) platelets as a response to various stimuli. What antibodies would you recommend for flow cytometry?
02 February 2014 5,688 6 View
I want to identify bovine neutrophils in blood samples based on a surface marker, and not just by forward/side scatter. A few articles mention Mab IL-A 110 as a neutrophil-specific marker, but the...
01 January 2014 3,378 1 View