Ina Laura Pieper

7 Questions 24 Answers 0 Followers

Questions related from Ina Laura Pieper

FACSAria bad for absolute cell counts?

I recently heard that the FACSAria is not a good instrument for the evaluation of absolute counts using beads because of the construction of the flow cell and dual velocity that exists within the...

02 February 2016 6,858 2 View

What do you think of this centrifugation protocol for preparing plasma samples for microparticle analysis by flow cytometry from peripheral blood?

Peripheral blood tubes are immediately inverted 10 times and centrifuged at 4,200 x g, 7 min, with brake. The supernatant plasma is transferred to a new tube and centrifuged at 13,000 x g, 5 min...

01 January 2015 4,890 4 View

Wharton's jelly single cell suspension?

I would like to prepare a single cell suspension of Wharton's jelly for flow cytometry. Any protocol recommendations?

06 June 2014 7,672 2 View

What is the standard protocol to induce bovine tissue factor expression using LPS?

I'd like to trial an antibody targeting tissue factor expression on bovine monocytes and I'm looking for the standard LPS-treatment to use as a positive control. What's your experience?

03 March 2014 2,376 5 View

Can someone recommend a multicolour immune cell activation flow cytometry panel?

I'm looking to monitor immune cell activation (neutrophils, monocytes, T/B/NK-cells) using multicolour flow cytometry and was wondering if anyone can recommend already established panels?

02 February 2014 3,385 11 View

What's the best activation marker antibodies to use for pig platelets?

I'm planning to investigate the activation status of porcine (pig) platelets as a response to various stimuli. What antibodies would you recommend for flow cytometry?

02 February 2014 5,689 6 View

Where can I buy Mab IL-A 110 targeting bovine neutrophils?

I want to identify bovine neutrophils in blood samples based on a surface marker, and not just by forward/side scatter. A few articles mention Mab IL-A 110 as a neutrophil-specific marker, but the...

01 January 2014 3,379 1 View