What is the cause of the banding obtained after purifying the PCR product with ExoSAPIT?

Hello We are working on a PCR product with an approximate size between 5000 and 7000 bp, we want to use it as a positive internal control, since we have sequenced it and it corresponds to the...

17 February 2021 2,896 3 View

CAT assay in microplate?

I'd like to know if anyone can provide me with a protocol for traditional catalase enzymatic assay in a 96 well microplate, for cell line and/or C.elegans spectrophotometric measurements. Thanks!

11 February 2021 7,273 3 View

GST assay in microplate?

I'd like to know if anyone can provide me with a protocol for traditional GST enzymatic assay (with GSH and CDNB as substrates) in a 96 well microplate, for cell line and/or C.elegans...

11 February 2021 1,328 3 View

Any good reference that links economic/industrial policy to changed agent behavior?

Usually industrial policy is interpreted as changing economic structure from in favor of more productive/advanced economic activities. But would like to read something that discusses the...

31 December 2020 3,199 3 View

How to calculate the necessary sample size for a subgroup descriptive analysis?

I want to do a descriptive analysis using the World Values Survey dataset which has an N=1200. However, even thought I have searched a lot, I haven't found the methodology or a tool to calculate...

17 November 2020 1,505 4 View

What is the relationship between plant hormones and plant allometry?

Hi, i'm looking for bibliographic references that explains the relationship between plant hormones and plant allometry (relation between the size of an organism and aspects of its physiology and...

21 September 2020 9,653 6 View

Why does the value of fresh and dry weight change in a plant?

In an experiment I found that the fresh weight of the root was less than that of the stem, however when I found the dry weight, the weight of the stem was less than that of the root. Why are these...

20 September 2020 2,269 16 View

Has anynone successfully used NEB's TtAgo (Thermus Thermophilus Argonaute) for any type of application?

I have been trying to use NEB's TtAgo (https://www.neb.com/products/m0665-ttago) to linearize supercoiled plasmids, following NEB's recommendations, but I haven't had any success yet (19/19 failed...

09 September 2020 2,112 1 View

What does it really mean to "hold other variables constant" in a regression?

To illustrate my point I present you an hypothetical case with the following equation: wage=C+0.5education+0.3rural area ( Where the variable "education" measures the number of years of...

10 July 2020 2,839 12 View

Is there a NO corrosion application for a linear polarization resistance?

Is there a NO corrosion application for a linear polarization resistance? Any example? And what is the technical justification for using +/- 10 mV as a signal of disturbance in linear...

09 June 2020 3,825 4 View

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02 March 2021 3,060 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

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28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Has anyone experienced changes in growth for cell culture when purchasing media from different companies?

I recently switched the company where we buy our DMEM/F12 and i have noticed a signficant change in growth for my human olfactory neurosphere cell lines

24 February 2021 2,268 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

Any easy method of analyzing vitamin D in foods by HPLC?

Looking for method of extracting and analyzing vitamin D in foods by HPLC

24 February 2021 7,524 7 View