Santiago Elías Charif

10 Questions 10 Answers 0 Followers

Questions related from Santiago Elías Charif

Presence of comets in control samples?

Hi! I am performing an alkaline comet assay (CHO K1 cells) by setting a protocol based on several publications. I've followed every step and recommendations, but I still see comets in my control...

28 October 2022 7,871 3 View

Any DNA Laddering (Fragmentation) protocol?

I wonder if anyone could provide me a detailed protocol or related publication for DNA Laddering (for cell lines). I know there are a couple of questions about it here, and reviews are diverse and...

26 May 2022 6,687 3 View

Non-fluorescent ROS detection in cells?

I want to detect ROS production in cells but spectrophotometrically, using an UV-visible spectrophotometer. As where I work we don't have a fluorescence reader (as used with the h2DCFDA probe), I...

01 November 2021 5,420 4 View

Vitamin E and vitamin C concentrations as antioxidants in cell culture?

I want to test antioxidant activity of a compound in neuronal cell culture and compare it with vitamin E (or Trolox) and vitamin C effects. Which concentrations of each would your recommend? Or...

02 October 2021 4,753 3 View

Microplate assay for spectrophotometric catalase activity determination?

I've read several works that assess catalase assay from samples by spectrophotometry based on Aebi (1984). Based on this, some works employ a microplate for this determination, and this is what I...

10 September 2021 668 4 View

¿Oxidative stress markers antibodies for C.elegans?

I wonder if anyone can recommend me primary antibodies for WB (and IF) with C.elegans. I'm planning to acquire the following antibodies but I'm still doubtful, as I am a begginer in everything...

26 February 2021 1,304 0 View

CAT assay in microplate?

I'd like to know if anyone can provide me with a protocol for traditional catalase enzymatic assay in a 96 well microplate, for cell line and/or C.elegans spectrophotometric measurements. Thanks!

12 February 2021 7,462 3 View

GST assay in microplate?

I'd like to know if anyone can provide me with a protocol for traditional GST enzymatic assay (with GSH and CDNB as substrates) in a 96 well microplate, for cell line and/or C.elegans...

12 February 2021 1,516 3 View

300 micron thick brain slice mounting?

Hi everyone, I'm trying to mount a 300 um-thick coronal brain slice after immunofluorescence onto a gelatin-coated slide. The problem is that the coverslip is lifted by the tissue, the mounting...

13 June 2018 6,176 7 View

Can anyone recommend a good primary antibody against melatonin receptor 1A and 1B, that works in several rodent species in WB and immunofluorescence?

I've tried SCBT's sc-13180 and sc-13177 and didn't work; Abcam's ab192662 and ab87639 worked poorly, in the animal model I'm working with (vizcacha - Lagostomus maximus). I'm from Argentina.

01 January 1970 1,783 2 View