How do mycoplasmas enter my cell cultures? http://www.bionique.com/mycoplasma-resources/faq/mycoplasma-enter-cell-cultures.html

How do mycoplasmas enter my cell cultures? http://www.bionique.com/mycoplasma-resources/faq/mycoplasma-enter-cell-cultures.html

03 April 2012 6,269 1 View

Video 1 Introduction to cell culture http://media.invitrogen.com.edgesuite.net/Cell-Culture/videos/CellCultureBasics.html?CID=ccbvid1

Video 1 Introduction to cell culture http://media.invitrogen.com.edgesuite.net/Cell-Culture/videos/CellCultureBasics.html?CID=ccbvid1

03 April 2012 8,162 0 View

Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections

Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections

07 August 2011 644 0 View

You can find more information about mouse strains here.

You can find more information about mouse strains here.

07 August 2011 3,363 2 View

IHC Protocol Summary

IHC Protocol Summary http://www.abcam.com/ps/pdf/protocols/immunostaining_at_a_glance.pdf

04 May 2011 9,781 0 View

FIXATION AND PERMEABILIZATION IN IHC/ICC

http://www.abcam.com/ps/pdf/protocols/fixation_permeabilization.pdf Fixation and permeabilization for IHC and ICC Tips on various fixation and permeabilization methods in IHC and ICC View a...

04 May 2011 3,525 0 View

FIXATION AND PERMEABILIZATION IN IHC/ICC

http://www.abcam.com/ps/pdf/protocols/fixation_permeabilization.pdf Fixation and permeabilization for IHC and ICC Tips on various fixation and permeabilization methods in IHC and ICC View a...

04 May 2011 8,263 0 View

Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections

Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections View Full Protocol The following protocol has been developed and optimized by R&D Systems IHC/ICC...

03 April 2011 9,997 0 View

Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections

Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections View Full Protocol The following protocol has been developed and optimized by R&D Systems IHC/ICC...

02 March 2011 6,840 0 View

Adipogenic Differentiation of Human/Mouse Mesenchymal Stem Cells

Adipogenic Differentiation of Human/Mouse Mesenchymal Stem Cells Mesenchymal stem cells (MSCs) are functionally defined by their capacity to self renew and their ability to differentiate into...

02 March 2011 5,735 0 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View