How do mycoplasmas enter my cell cultures?
http://www.bionique.com/mycoplasma-resources/faq/mycoplasma-enter-cell-cultures.html
Add mercaptoethanol
Video - Sterile technique http://media.invitrogen.com.edgesuite.net/Cell-Culture/videos/SterileTechnique.html?CID=ccbvid2
03 April 2012 1,126 0 View
Video 1 Introduction to cell culture http://media.invitrogen.com.edgesuite.net/Cell-Culture/videos/CellCultureBasics.html?CID=ccbvid1
03 April 2012 8,262 0 View
Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
07 August 2011 761 0 View
You can find more information about mouse strains here.
07 August 2011 3,471 2 View
IHC Protocol Summary http://www.abcam.com/ps/pdf/protocols/immunostaining_at_a_glance.pdf
04 May 2011 9,898 0 View
http://www.abcam.com/ps/pdf/protocols/fixation_permeabilization.pdf Fixation and permeabilization for IHC and ICC Tips on various fixation and permeabilization methods in IHC and ICC View a...
04 May 2011 3,626 0 View
04 May 2011 8,409 0 View
Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections View Full Protocol The following protocol has been developed and optimized by R&D Systems IHC/ICC...
03 April 2011 10,118 0 View
Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections View Full Protocol The following protocol has been developed and optimized by R&D Systems IHC/ICC...
02 March 2011 7,006 0 View
Adipogenic Differentiation of Human/Mouse Mesenchymal Stem Cells Mesenchymal stem cells (MSCs) are functionally defined by their capacity to self renew and their ability to differentiate into...
02 March 2011 5,845 0 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates
03 August 2024 3,505 6 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View