IHC Protocol Summary

Seyed Mahmoud Hashemi @Seyed_Mahmoud_Hashemi

05 May 2011 0 10K Report

http://www.abcam.com/ps/pdf/protocols/immunostaining_at_a_glance.pdf

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Video - Sterile technique http://media.invitrogen.com.edgesuite.net/Cell-Culture/videos/SterileTechnique.html?CID=ccbvid2

03 April 2012 910 0 View

How do mycoplasmas enter my cell cultures? http://www.bionique.com/mycoplasma-resources/faq/mycoplasma-enter-cell-cultures.html

03 April 2012 6,269 1 View

Video 1 Introduction to cell culture http://media.invitrogen.com.edgesuite.net/Cell-Culture/videos/CellCultureBasics.html?CID=ccbvid1

03 April 2012 8,162 0 View

Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections

07 August 2011 644 0 View

You can find more information about mouse strains here.

07 August 2011 3,363 2 View

FIXATION AND PERMEABILIZATION IN IHC/ICC

http://www.abcam.com/ps/pdf/protocols/fixation_permeabilization.pdf Fixation and permeabilization for IHC and ICC Tips on various fixation and permeabilization methods in IHC and ICC View a...

04 May 2011 3,525 0 View

FIXATION AND PERMEABILIZATION IN IHC/ICC

http://www.abcam.com/ps/pdf/protocols/fixation_permeabilization.pdf Fixation and permeabilization for IHC and ICC Tips on various fixation and permeabilization methods in IHC and ICC View a...

04 May 2011 8,263 0 View

Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections

Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections View Full Protocol The following protocol has been developed and optimized by R&D Systems IHC/ICC...

03 April 2011 9,997 0 View

Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections

Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections View Full Protocol The following protocol has been developed and optimized by R&D Systems IHC/ICC...

02 March 2011 6,840 0 View

Adipogenic Differentiation of Human/Mouse Mesenchymal Stem Cells

Adipogenic Differentiation of Human/Mouse Mesenchymal Stem Cells Mesenchymal stem cells (MSCs) are functionally defined by their capacity to self renew and their ability to differentiate into...

02 March 2011 5,735 0 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

I am looking for an antibody to Arc protein for use in immunohistochemistry with rhesus monkey brain tissue?

Looking for a well-validated antibody to detect Arc protein expression in paraformaldehyde fixed brain tissue using immunohistochemistry. Ideally this antibody would have been published on using...

07 February 2021 8,457 1 View

How should I go about evaluating cytoplasmic and nuclear Arginase-1 activity?

Hello! I've stained some bright field Arginase-1 Hematoxylin slides, but I'm having a hard time finding any computed histology predicates for evaluating the intensity of the cytoplasm verses the...

03 February 2021 3,039 1 View

Antibody dilution for IHQ in OCT frozen tissue is more similar to IHC in Paraffin-embedded samples or to Immunocytochemistry?

31 January 2021 8,678 3 View

Any tips on Fab blocking of mouse tissue?

Hi, everyone, I am working on immunostaining of bone marrow and growth plates of mice. And I have to use an mouse monoclonal Ab to do this staining. However, with only secondary goat anti-mouse...

21 January 2021 9,826 3 View

Antigen retrieval for neural cell marker on frozen brain tissue?

Hi, I'm having problems with the Recombinant Anti-MAP2 antibody from Abcam (Recombinant Anti-MAP2 antibody [EPR19691] (ab183830)) antibody on my frozen, 4%PFA perfused, mice brain tissue. I...

10 January 2021 4,002 5 View

How to approach quantification of cytoplasmic immunofluorescent signal in adipocytes?

Any ideas on how to quantify a non-nuclear immunofluorescent signal in adipocytes? I had the idea to do a watershed on inverted image to "locate" the center of the empty space where lipid droplets...

05 January 2021 5,420 1 View

Can you recommend a protocol for immunohistochemistry of mouse brain sections using anti-MAP2?

Current protocol does not give clear images.

04 January 2021 1,614 3 View

I need to know about immunohistochemistry steps?

Immunohistochemistry staining. Tissue Fixation during IHC. Using DAB. blocking step. controls group.

23 December 2020 5,768 2 View

ISO antibody against BFP for FFPE IHC?

Suggestions and recommendations welcome. Any usage/protocol experience would also be helpful/appreciated. Cheers

22 December 2020 599 3 View