Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
http://www.rndsystems.com/ihc_detail_objectname_chromogenic_ihc_paraffin_tissue.aspx
Video - Sterile technique http://media.invitrogen.com.edgesuite.net/Cell-Culture/videos/SterileTechnique.html?CID=ccbvid2
03 April 2012 1,126 0 View
How do mycoplasmas enter my cell cultures? http://www.bionique.com/mycoplasma-resources/faq/mycoplasma-enter-cell-cultures.html
03 April 2012 6,363 1 View
Video 1 Introduction to cell culture http://media.invitrogen.com.edgesuite.net/Cell-Culture/videos/CellCultureBasics.html?CID=ccbvid1
03 April 2012 8,262 0 View
You can find more information about mouse strains here.
07 August 2011 3,471 2 View
IHC Protocol Summary http://www.abcam.com/ps/pdf/protocols/immunostaining_at_a_glance.pdf
04 May 2011 9,898 0 View
http://www.abcam.com/ps/pdf/protocols/fixation_permeabilization.pdf Fixation and permeabilization for IHC and ICC Tips on various fixation and permeabilization methods in IHC and ICC View a...
04 May 2011 3,626 0 View
04 May 2011 8,409 0 View
Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections View Full Protocol The following protocol has been developed and optimized by R&D Systems IHC/ICC...
03 April 2011 10,118 0 View
Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections View Full Protocol The following protocol has been developed and optimized by R&D Systems IHC/ICC...
02 March 2011 7,006 0 View
Adipogenic Differentiation of Human/Mouse Mesenchymal Stem Cells Mesenchymal stem cells (MSCs) are functionally defined by their capacity to self renew and their ability to differentiate into...
02 March 2011 5,845 0 View
I am doing immunohistochemistry of the c-Fos protein and I have a budget problem. For context, the experiment has been done over 3 years prior to my arrival and I have 40 brains done and 100 left....
29 July 2024 5,961 2 View
Hello everyone, A collaborator sent us his protocol in order to be able to use his immunohistochemistry kits. But one step contains PBS + 0.3% H2O2 + 0.1% sodium azide. Knowing the dangerousness...
23 July 2024 6,314 3 View
We have mice brains that were over-fixed due to old PFA used during perfusion. Thus, the synapses are no longer being labeled by the Synaptophysin (SY38) mAB. which works perfectly every other...
17 July 2024 7,767 3 View
I'm looking to immunostain some brain tissue collected previously. The animal was *not* perfused prior to tissue collection. Will the presence of blood interfere with all staining methods...
14 July 2024 177 4 View
Hi all, I would like to know if it is possible to use Bovine Serum Albumin (BSA) in my blocking solution for immunofluorescence muscle fiber typing. The primary antibodies' host is mouse. The...
07 July 2024 7,967 3 View
In the context of doctoral research, I utilized immunohistochemical (IHC) analysis to quantify the expression levels of the Ki-67 proliferation marker. While the application of this test and...
25 June 2024 7,825 2 View
Hello! I've recently been trying to optimise immunofluorescent staining of mouse meningeal tissue, and I'm having trouble mounting it just because it's so thick. We use prolong to mount and...
05 June 2024 624 2 View
I need to do immunohistochemistry in mouse brain, particularly in neurotensin-cre mouse line.
22 May 2024 3,565 7 View
Hi all, I've processed my own IHC for many years and never run into this problem before, but the last few times I've tried staining, I've had the same issue. I'm wondering if it's a reagent...
08 May 2024 3,979 1 View
I have been using Shandon Sequenza for the last two years with no problems. Lately i have noticed small spots under a microscope that have not been stained by DAB (only hematoxylin as a contrast...
28 April 2024 2,530 1 View