Using DDM (n-Dodecyl-B-D-Maltoside) in co-immunoprecipitation?

11 May 2022 1 5K Report

Hi everyone,

I am trying to optimise a co-IP from liver tissue. For other applications, we routinely use 1% DDM in the lysis buffer - does anyone have experience with it in co-IP using Dynabeads Protein A magnetic beads?

Johanna Wagnerberger

Hello Chiara!

Did you figure out a good protocol for this?

I am using 1.5% DDM in my lysis buffer, but the first IP with the Dynabeads led to a pretty strong unspecific band on the gel.

I only had 0.05% DDM in the wash buffer. I thought it might be enough since this is well over the CMC of DDM, but maybe it wasn't?

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