I bought from Epigentek the EpiQuik DNMT Activity/Inhibition ELISA Easy Kit P-3139-48 and the CA capture antibody must be stored at 4°C but I put in at - 20 °C.
I bought from Epigentek the HDAC Activity/Inhibition Direct Assay kit P-4034-48 and the HO4 capture antibody must be stored at 4 °C but I put it at - 20 °C.
Does it happen also to someone? Is it possible to use it? Do I have to remove from freezer and put in fridge?
Thanks if someone can help me
Hello Chiara Rucci
Yes, it is possible to use it.
The antibodies should not get damaged with a single freeze-thaw cycle. So, you may remove the antibodies as well as the kits from the -20°C freezer, thaw them, mix the antibodies as well as the kit reagents gently (please do not vortex), and place the kits and the antibodies in the refrigerator at 4°C.
Best.
I agree with Malcolm, that you can use it. However, to be in the safe side, I would suggest that you contact Epigentek and ask for their advice before you engaged in serious work with your reagents. Best of luck.
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