Johanna Wagnerberger

4 Questions 6 Answers 0 Followers

Questions related from Johanna Wagnerberger

Why is Tris-HCl much more popular than PBS/HEPES for cell lysis buffers?

Hello! I am preparing a protocol for cell lysis of tissue samples. I want native conformation of as many proteins as possible and would therefore like to have good pH control. In most protocols,...

25 May 2023 3,366 6 View

Optimal buffer for total native protein extract from tissue?

Hello! I am working on a protocol to extract as many native proteins as possible from brain tissue. The end goal is to use the supernatant of the lysate in immunoprecipitation against some...

09 May 2023 7,928 1 View

How to remove "sticky" heat shock protein contamination from monoclonal antibodies?

Hello! I have produced monoclonal antibodies by transfecting HEK293 cells and purifying then using a protein A column. To check for purity I ran several SDS-PAGEs and saw faint bands at ca 70...

12 April 2023 2,481 5 View

SEC curve looks good, but SDS-PAGE is bad?

Hello! I've been producing some monoclonal antibodies with an unknown target. As a quality control, I've been running a reducing SDS-PAGE on 12% gel (to check for contaminants and control the...

19 January 2023 9,324 2 View