Chiara Giacomelli

4 Questions 2 Answers 0 Followers

Questions related from Chiara Giacomelli

Using DDM (n-Dodecyl-B-D-Maltoside) in co-immunoprecipitation?

Hi everyone, I am trying to optimise a co-IP from liver tissue. For other applications, we routinely use 1% DDM in the lysis buffer - does anyone have experience with it in co-IP using Dynabeads...

11 May 2022 4,617 1 View

Any easy way to query the promoter of a gene for TF binding sites?

Is there any web tool to use where you can insert the gene ID of Your Favourite Gene, and the result is a list of putative/validated TF binding the promoter of YFG? Without having to search for...

15 April 2016 9,509 5 View

SiRNA control transfection on top of siRNA against target gene to equalize concentration in phenotypic assay?

Considering a phenotypic assay (proliferation/viability), would you transfect together with the target gene siRNA also negative ctrl siRNAs to achieve the same concentration as the multiple target...

08 October 2014 6,321 3 View

Any tips and tricks when analysing a promoter region?

I never worked on promoter regions and we are currently trying to characterize one. We started from ENCODE data on chromatine state to identify the area of interest and will probably go for some...

18 June 2014 9,605 2 View