Chiara Giacomelli

3 Questions 2 Answers 0 Followers

Questions related from Chiara Giacomelli

Using DDM (n-Dodecyl-B-D-Maltoside) in co-immunoprecipitation?

Hi everyone, I am trying to optimise a co-IP from liver tissue. For other applications, we routinely use 1% DDM in the lysis buffer - does anyone have experience with it in co-IP using Dynabeads...

11 May 2022 4,578 1 View

Any easy way to query the promoter of a gene for TF binding sites?

Is there any web tool to use where you can insert the gene ID of Your Favourite Gene, and the result is a list of putative/validated TF binding the promoter of YFG? Without having to search for...

15 April 2016 9,478 5 View

SiRNA control transfection on top of siRNA against target gene to equalize concentration in phenotypic assay?

Considering a phenotypic assay (proliferation/viability), would you transfect together with the target gene siRNA also negative ctrl siRNAs to achieve the same concentration as the multiple target...

08 October 2014 6,256 3 View