Using alternative filter paper membrane - any thoughts?

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Is there an article or book you would recommend for determining the P-T conditions of olivine minerals in pallasite meteorites?

Additionally, what specific conditions may occur during the crystallization process of minerals in the formation of meteorites?

12 February 2024 6,784 4 View

IDoes anyone know how I can access the ICRG data?

Hello, I am doing my research on the relationship between corruption in the context of macroeconomic indicators and I need corruption index data from ICRG. Since I need data from 1985 to 2022,...

04 June 2023 3,692 0 View

Can anyone help me with the reason for the fuzzy gel electrophoresis?

With below protocol we got fuzzy bands like in attachment. Can anyone give me an idea what's the problem with this protocol? Insect sample for extraction. 1,7 % gel 35 running time 120 V TAE Buffer

15 November 2022 8,741 2 View

How can we estimate the reliability of a single-item measure?

Dear all, I wonder how can I test the reliability of a single-item measure that I have used in my paper. I have found equation 9 of Heise (1969; https://www.jstor.org/stable/pdf/2092790.pdf);...

22 November 2021 7,943 2 View

Exo or Endo polygalacturonase ?

I am working on polygalacturonases. There are two type of polygalacturonases one is Exo and other is Endo. I want to learn that which one is mosth important for industrial usage ?

04 December 2018 4,383 1 View

How can I compute an inverse of a large and sparse matrix approximately?

Inverse of matrices are needen in both nonlinear and linear preconditioing (see Additive Shcwarz). Since it is too costly, I am searching how to calculte the inverse of a matrix in an approximate way.

17 October 2017 2,346 1 View

Has anyone noticed a toxic effect of KnockOut Serum Replacement on MEF feeder cells?

%100 confluent MEF feeder cells in 6-well culture dish got shrinked and showed a phenotype looks like under a serious stress condition after we changed the medium from MEF medium (including...

06 September 2015 8,179 3 View

Does anybody know what is the initial step and use output for several second in ANSYS?

07 August 2015 2,330 3 View

How can I produce recombinant ENZYME ?

I need to produce Oxalate decarboxylase enzyme as a recombinant enzyme. I choosed my source organisim named bacillus subtilis 168 designed my primers (with doubt) and I want to express in pET-SUMO...

28 January 2015 1,530 4 View

Using different secondary antibody on the same membrane?

I have two different secondary antibodies. I use one of them on nitrosellulose membrane and I need to use the other one. Do I need to use primary antibody again or can I use secondary antibody...

06 February 2014 8,994 2 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

Does anyone know what might be causing the smeared bands on my western blot?

I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...

10 August 2024 7,480 3 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

How are iso-frequency contours plotted?

Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...

07 August 2024 1,937 1 View

How to prepare the nanoparticle treated fungal sample for Environmental SEM analysis?

A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.

07 August 2024 5,307 1 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View