Gnana Ajay

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Questions related from Gnana Ajay

Using 2^-(ΔΔCt) or 2^(ΔΔCt) for qpcr analysis?

I am comparing two different cell lines (LN229 and HA), LN229 as target sample and HA as reference sample. If I do the 2^-(ΔΔCt) method as LN229 - HA some genes get a fold change value of 41 and...

02 January 2024 9,062 2 View

To check specificity of your primers by ncbi blast, we find identity percentage, query cover and E-value as the parameter to be taken care off?

Hello, To check specificity of your primers by ncbi blast, we find identity percentage, query cover and E-value as the parameter to be taken care off. I know that query cover is important but i...

14 December 2023 6,966 1 View