Has anyone ever used viruses to express fluorescent proteins in living insects?
I used NPV virus which carried the target gene to transfect or infect silkworm Bombyx mori.
Hello, can anyone suggest me a free bioinformatic tool to calculate/simulate the vibrational spectra of common simple molecules (i.e. C-6, C-7 alcohols, aldehydes, and ketones)?
09 October 2014 5,813 2 View
I am looking for markers such as c-fos or phospho-CREB that could reveal neuronal activity in honeybee. So far I have found only d-fos (or kayak, the drosophila homolog) but there is no good...
08 September 2013 1,442 19 View
I am searching for markers alternative to "SYNAPSIN" to stain insect synaptic terminals. Any ideas? Possibly a rabbit antibody.
08 September 2013 1,675 2 View
I am looking for a baculovirus vector expressing calcium reporters in neurons. I know some companies prepare custom viral vectors and sell aliquotes of the most commercial ones. Can anyone...
06 July 2013 9,885 4 View
We have been thinking about all IHC and ICC data that we have collected during our PhD and that will never be published. In fact, only a minor portion of all experimental work have been published...
04 May 2013 5,474 4 View
I am trying to perform immunohistochemistry on the whole brain of bees using antibodies anti-Repo and anti-Elav (from DSHB, Iowa) but with very little result. Has anyone experience with one of...
02 March 2013 500 0 View
Is it possible to do some kind of iv injection in honey bees in order to deliver a molecule systemically?
09 October 2012 4,406 4 View
Invertebrates have also glial cells but they are a bit "different" and less specialized than the one we can find in mammals. Does anyone know if markers such as GFAP, EAAT-1, EAAT-2, GLUTAMINE...
09 October 2012 2,430 8 View
I would like to measure a possible change in synaptic coverage by astrocytes upon memory formation. Do you think there is some way to measure it by IHC and optic microscopy (mainly 2-photon...
09 October 2012 8,437 5 View
I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?
11 August 2024 3,115 1 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...
05 August 2024 3,290 1 View
All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.
01 August 2024 589 4 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View
I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...
28 July 2024 366 3 View
I designed 2 dual hybridization probes for my single SNP. where I attach my fluorophore 5'-FAM-nnnn-BHQ1-3', and 5'-HEX-nnnnnn-BHQ2-3'. I need to run it on the MIC RT PCR machine by Biomolecular...
28 July 2024 5,658 0 View
I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...
28 July 2024 3,178 3 View
I'm having problems with ion exchange chromatography. After applying my sample (a fluorescent protein), a preferential path inevitably forms. The matrix I use is DEAE-Sephacel. Could someone...
27 July 2024 6,605 0 View