04 April 2013 1 10K Report

Has anyone ever used viruses to express fluorescent proteins in living insects?

More Marco Paoli's questions See All
Is there a free tool to calculate/simulate the vibrational spectra of common molecules?

Hello, can anyone suggest me a free bioinformatic tool to calculate/simulate the vibrational spectra of common simple molecules (i.e. C-6, C-7 alcohols, aldehydes, and ketones)?

09 October 2014 5,735 2 View

Any known marker for neuronal activity in insects?

I am looking for markers such as c-fos or phospho-CREB that could reveal neuronal activity in honeybee. So far I have found only d-fos (or kayak, the drosophila homolog) but there is no good...

08 September 2013 1,373 19 View

Any good markers for insect synapses?

I am searching for markers alternative to "SYNAPSIN" to stain insect synaptic terminals. Any ideas? Possibly a rabbit antibody.

08 September 2013 1,591 2 View

Where to buy viral vectors?

I am looking for a baculovirus vector expressing calcium reporters in neurons. I know some companies prepare custom viral vectors and sell aliquotes of the most commercial ones. Can anyone...

06 July 2013 9,816 4 View

Regarding an unpublished IHC/ICC data and results database?

We have been thinking about all IHC and ICC data that we have collected during our PhD and that will never be published. In fact, only a minor portion of all experimental work have been published...

04 May 2013 5,406 4 View

Has anyone ever used the anti-Repo or anti-Elav antibodies for IHC?

I am trying to perform immunohistochemistry on the whole brain of bees using antibodies anti-Repo and anti-Elav (from DSHB, Iowa) but with very little result. Has anyone experience with one of...

02 March 2013 430 0 View

"Intra venous" injection in honeybees, is it possible?

Is it possible to do some kind of iv injection in honey bees in order to deliver a molecule systemically?

09 October 2012 4,335 4 View

Does GFAP antibody work in Drosophila?

Invertebrates have also glial cells but they are a bit "different" and less specialized than the one we can find in mammals. Does anyone know if markers such as GFAP, EAAT-1, EAAT-2, GLUTAMINE...

09 October 2012 2,366 8 View

How to measure an increase of astrocytes' synaptic coverage?

I would like to measure a possible change in synaptic coverage by astrocytes upon memory formation. Do you think there is some way to measure it by IHC and optic microscopy (mainly 2-photon...

09 October 2012 8,362 5 View

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What are the countries most affected by extreme rainfall as a result of climate change?

Increased heat, drought and insect outbreaks, all associated to climate change, have increased wildfires. Decreasing water supplies, declined agricultural yields, health impacts in cities owing to...

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How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

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Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

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Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

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