I am trying to perform immunohistochemistry on the whole brain of bees using antibodies anti-Repo and anti-Elav (from DSHB, Iowa) but with very little result. Has anyone experience with one of these antibodies (even if not in bees)?
Hello, can anyone suggest me a free bioinformatic tool to calculate/simulate the vibrational spectra of common simple molecules (i.e. C-6, C-7 alcohols, aldehydes, and ketones)?
09 October 2014 5,813 2 View
I am looking for markers such as c-fos or phospho-CREB that could reveal neuronal activity in honeybee. So far I have found only d-fos (or kayak, the drosophila homolog) but there is no good...
08 September 2013 1,442 19 View
I am searching for markers alternative to "SYNAPSIN" to stain insect synaptic terminals. Any ideas? Possibly a rabbit antibody.
08 September 2013 1,675 2 View
I am looking for a baculovirus vector expressing calcium reporters in neurons. I know some companies prepare custom viral vectors and sell aliquotes of the most commercial ones. Can anyone...
06 July 2013 9,885 4 View
We have been thinking about all IHC and ICC data that we have collected during our PhD and that will never be published. In fact, only a minor portion of all experimental work have been published...
04 May 2013 5,474 4 View
Has anyone ever used viruses to express fluorescent proteins in living insects?
03 April 2013 9,721 1 View
Is it possible to do some kind of iv injection in honey bees in order to deliver a molecule systemically?
09 October 2012 4,406 4 View
Invertebrates have also glial cells but they are a bit "different" and less specialized than the one we can find in mammals. Does anyone know if markers such as GFAP, EAAT-1, EAAT-2, GLUTAMINE...
09 October 2012 2,430 8 View
I would like to measure a possible change in synaptic coverage by astrocytes upon memory formation. Do you think there is some way to measure it by IHC and optic microscopy (mainly 2-photon...
09 October 2012 8,437 5 View
Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...
04 August 2024 4,725 2 View
I am doing immunohistochemistry of the c-Fos protein and I have a budget problem. For context, the experiment has been done over 3 years prior to my arrival and I have 40 brains done and 100 left....
29 July 2024 5,961 2 View
Hello everyone, A collaborator sent us his protocol in order to be able to use his immunohistochemistry kits. But one step contains PBS + 0.3% H2O2 + 0.1% sodium azide. Knowing the dangerousness...
23 July 2024 6,314 3 View
Hi, I am a master's student who studies inflammation. I'm planning to extract RNA from the Vagal nerve or Dorsal Ganglion roots, but I got a question. It would be only a few 50ug per each nerve,...
23 July 2024 6,783 1 View
Kindly confirm how to distinguish glia cells and neurons using cresyl violet staining. Also, using cresyl violet nissl staining how to identify the apoptotic neurons ? Does this stain stains...
18 July 2024 2,420 0 View
We have mice brains that were over-fixed due to old PFA used during perfusion. Thus, the synapses are no longer being labeled by the Synaptophysin (SY38) mAB. which works perfectly every other...
17 July 2024 7,767 3 View
Unlike humans, it is believed that birds do not have a symbolic language system that can be reduced to words (Berwick et al. 2012). But they do have a nervous system that can generate sequences...
14 July 2024 9,852 0 View
I'm looking to immunostain some brain tissue collected previously. The animal was *not* perfused prior to tissue collection. Will the presence of blood interfere with all staining methods...
14 July 2024 177 4 View
I need help for find a subject for PhD in insect physiology
12 July 2024 8,227 1 View
I want to know what are the relationship that happen between insect biodiversity and durian tree
10 July 2024 8,230 1 View