Is there a free tool to calculate/simulate the vibrational spectra of common molecules?

Hello, can anyone suggest me a free bioinformatic tool to calculate/simulate the vibrational spectra of common simple molecules (i.e. C-6, C-7 alcohols, aldehydes, and ketones)?

09 October 2014 5,735 2 View

Any known marker for neuronal activity in insects?

I am looking for markers such as c-fos or phospho-CREB that could reveal neuronal activity in honeybee. So far I have found only d-fos (or kayak, the drosophila homolog) but there is no good...

08 September 2013 1,373 19 View

Any good markers for insect synapses?

I am searching for markers alternative to "SYNAPSIN" to stain insect synaptic terminals. Any ideas? Possibly a rabbit antibody.

08 September 2013 1,591 2 View

Where to buy viral vectors?

I am looking for a baculovirus vector expressing calcium reporters in neurons. I know some companies prepare custom viral vectors and sell aliquotes of the most commercial ones. Can anyone...

06 July 2013 9,816 4 View

Regarding an unpublished IHC/ICC data and results database?

We have been thinking about all IHC and ICC data that we have collected during our PhD and that will never be published. In fact, only a minor portion of all experimental work have been published...

04 May 2013 5,406 4 View

Use virus to express GFP in insects in vivo

Has anyone ever used viruses to express fluorescent proteins in living insects?

03 April 2013 9,653 1 View

"Intra venous" injection in honeybees, is it possible?

Is it possible to do some kind of iv injection in honey bees in order to deliver a molecule systemically?

09 October 2012 4,335 4 View

Does GFAP antibody work in Drosophila?

Invertebrates have also glial cells but they are a bit "different" and less specialized than the one we can find in mammals. Does anyone know if markers such as GFAP, EAAT-1, EAAT-2, GLUTAMINE...

09 October 2012 2,366 8 View

How to measure an increase of astrocytes' synaptic coverage?

I would like to measure a possible change in synaptic coverage by astrocytes upon memory formation. Do you think there is some way to measure it by IHC and optic microscopy (mainly 2-photon...

09 October 2012 8,362 5 View

What are the countries most affected by extreme rainfall as a result of climate change?

Increased heat, drought and insect outbreaks, all associated to climate change, have increased wildfires. Decreasing water supplies, declined agricultural yields, health impacts in cities owing to...

01 March 2021 7,168 9 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

How can I stain the somatodendritic part of inhibitory neurons in a hippocampal culture with high specificity?

I would like to distinguish the somatodendritic part of the inhibitory neurons in a hippocampal culture (where we expect to have 5-10% of GABAergic neurons). What are the best antibodies and...

23 February 2021 6,756 1 View

Please help in identification of this insect?

This insect is minute in size around 1 mm and not clearly visible with naked eye.

22 February 2021 5,907 11 View

The PC12 cell line characteristics?

Hello dear scientists, Does the undifferentiated PC12 cell line in terms of chromosome characteristics, proliferation, and drug reactions have the characteristics of cancer cells? Where is it...

21 February 2021 8,945 3 View

Purification of proteins from baculovirus-infected insect cells?

18 February 2021 6,665 6 View

What are alternatives for B27+ Supplement in neuronal cell culture?

Hello everybody In our iPSC-derived neuronal culture we use Maturationmedium, supplemented with B27+. As Thermo Fisher run out of stock and can deliver earliest in May, I wanted to ask whether...

16 February 2021 3,348 1 View

How to reduce debris with neuron isolation?

Hi there, I'm isolating neurons from PND1 guinea pigs and have noticed a bit of debris in the early days (this is day 4 plated) and a lot of variability between plates. Current protocol is...

14 February 2021 3,692 4 View