Use of HT in CDCHO media in GS selection system?

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Please suggest few trending & futuristic PhD Research Topics/ Gaps in Management (preferably Service Quality Management) in Higher Education in India?

Please suggest a few trending and futuristic PhD Research Topics/ Gaps in Management (preferably in Service Quality / Quality Management) in Education (preferably in Higher Education) in India....

08 April 2024 3,664 3 View

Does Nanodrop Concentration depend on the volume of the sample taken?

I checked the nanodrop conc of the plasmid sample and it was 1000 something. I cleaned the platform and the upper arm got mistakenly lowered and it still measured the conc around 41.Could be due...

14 January 2024 8,323 6 View

How can we introduce restriction sites in the plasmid using PCR?

If my plasmid doesn't have Ecor1 and sal1 but i need my plasmid to have those, I am aware that i can make primers with Ecor 1 and sal1 overhangs but how is that done from the beginning?

11 September 2023 2,510 3 View

Why is ampicillin and kenamycin antibiotic resistance incubation time are different? Why are sattelite colonies seen around the main colony ?

Does it have something to do with the fact that ampicillin acts only after the cells divide and kanamycin resistance has something to do with the ribosomes and then it inhibits the translation? I...

29 August 2023 4,997 1 View

Would it be wise to use the same plasmid for transfection after maxiprep if it got some water spilled in it?

Sgv was constructed and maxiprepped. The conc was 1965 ng/ul and accidently a few drops of nuclease free water got spilled in it. Can I still use the same SGV plasmid for transfection or should I...

29 August 2023 9,351 5 View

What went wrong?

I followed maxiprep protocol and the yield was as low as 5.9ng/ul. All the steps were followed and everything was taken care of. Is it because the cultures were a month old?

21 August 2023 2,773 5 View

In transient Transfection, how do we know for sure that gene of interest we transfected was not inserted into the host genome?

How do we decide which transfection it is? Does it depend on whether we used PEI buffer or electroporation?

07 August 2023 783 5 View

How do we know which restriction enzyme to choose for the digest in DGV and TGV?

We use Hind 111 is used almost every time but why? How many enzymes do we have to choose per experiment?

07 August 2023 8,176 2 View

What factors must be considered when choosing which binding partner to immobilize in biosensor in octet?

Some of us use Streptavidin coated plates.

06 August 2023 4,191 0 View

Why is it to hard to see the bright bands clearly at the bottom of the 1% agarose gel?

Does it have something to do with the concentration?

03 August 2023 1,547 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View

How can I get my Granzyme B flow cytometry stain to be consistent?

I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...

01 August 2024 7,677 2 View

Best mask design for metal deposition aiming build a Perovskite solar cell device?

What types of mask designs for metal deposition, to be used in a PVD system, are best suited for perovskite-based solar cells?

31 July 2024 4,835 3 View

How to culture and maintain Lx-2 cells?

Hi there! I recently started working on Lx-2 cells. When I thaw the cells, they start attaching within few hours but later in about 2 days they started rounding and dying. I used DMEM+2% FBS...

31 July 2024 3,893 0 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View