Yukti Kataria

13 Questions 20 Answers 0 Followers

Questions related from Yukti Kataria

Does Nanodrop Concentration depend on the volume of the sample taken?

I checked the nanodrop conc of the plasmid sample and it was 1000 something. I cleaned the platform and the upper arm got mistakenly lowered and it still measured the conc around 41.Could be due...

15 January 2024 8,277 6 View

How can we introduce restriction sites in the plasmid using PCR?

If my plasmid doesn't have Ecor1 and sal1 but i need my plasmid to have those, I am aware that i can make primers with Ecor 1 and sal1 overhangs but how is that done from the beginning?

12 September 2023 2,494 3 View

Would it be wise to use the same plasmid for transfection after maxiprep if it got some water spilled in it?

Sgv was constructed and maxiprepped. The conc was 1965 ng/ul and accidently a few drops of nuclease free water got spilled in it. Can I still use the same SGV plasmid for transfection or should I...

30 August 2023 9,340 5 View

Why is ampicillin and kenamycin antibiotic resistance incubation time are different? Why are sattelite colonies seen around the main colony ?

Does it have something to do with the fact that ampicillin acts only after the cells divide and kanamycin resistance has something to do with the ribosomes and then it inhibits the translation? I...

30 August 2023 4,969 1 View

What went wrong?

I followed maxiprep protocol and the yield was as low as 5.9ng/ul. All the steps were followed and everything was taken care of. Is it because the cultures were a month old?

22 August 2023 2,756 5 View

How do we know which restriction enzyme to choose for the digest in DGV and TGV?

We use Hind 111 is used almost every time but why? How many enzymes do we have to choose per experiment?

08 August 2023 8,152 2 View

In transient Transfection, how do we know for sure that gene of interest we transfected was not inserted into the host genome?

How do we decide which transfection it is? Does it depend on whether we used PEI buffer or electroporation?

08 August 2023 760 5 View

What factors must be considered when choosing which binding partner to immobilize in biosensor in octet?

Some of us use Streptavidin coated plates.

07 August 2023 4,172 0 View

Why is it to hard to see the bright bands clearly at the bottom of the 1% agarose gel?

Does it have something to do with the concentration?

04 August 2023 1,525 3 View

Why do we add media first and DMSO later in the cryopreservation of cells?

Why do we add media first and DMSO later?

03 August 2023 3,143 6 View

Why do we use different antibiotic resistance chosen in part vectors and destination vectors in golden gate assembly?

Hello. We did golden gate assembly where in part vectors we chose kanamycin resistance gene and in destination vector we chose ampicillin resistance. Why is that?

31 July 2023 5,452 3 View

Use of HT in CDCHO media in GS selection system?

Hello. I am using GS gene selection system in my research for CHO cells. My question is : when GSKO CHO cells are being transfected, why did my supervisor add 10% HT in the CD CHO media even...

27 July 2023 6,890 3 View

PiggyBac Transposon-mediated Gene Transfer

I have a question. We are doing transient transfection, shouldn't we use mRNA instead of DNA? because we do not want our fragment to be incorporated in the genomic DNA of host cell? isn't that...

01 January 1970 3,027 4 View