Yukti Kataria

5 Questions 9 Answers 0 Followers

Questions related from Yukti Kataria

How do we know which restriction enzyme to choose for the digest in DGV and TGV?

We use Hind 111 is used almost every time but why? How many enzymes do we have to choose per experiment?

08 August 2023 8,145 2 View

Why is it to hard to see the bright bands clearly at the bottom of the 1% agarose gel?

Does it have something to do with the concentration?

04 August 2023 1,516 3 View

Why do we add media first and DMSO later in the cryopreservation of cells?

Why do we add media first and DMSO later?

03 August 2023 3,137 6 View

Why do we use different antibiotic resistance chosen in part vectors and destination vectors in golden gate assembly?

Hello. We did golden gate assembly where in part vectors we chose kanamycin resistance gene and in destination vector we chose ampicillin resistance. Why is that?

31 July 2023 5,445 3 View

PiggyBac Transposon-mediated Gene Transfer

I have a question. We are doing transient transfection, shouldn't we use mRNA instead of DNA? because we do not want our fragment to be incorporated in the genomic DNA of host cell? isn't that...

01 January 1970 3,022 4 View