Hello Amera A.M Abdilnabi

You need not go for another culture medium. Use the same medium which you have been using ( with phenol red) for MTT assay. Though phenol red and the final formazan product absorption spectra overlap, and phenol red can interfere with the formazan absorbance reading, there is a way out.

In your case you will have to use acidified isopropanol to solubilize formazan crystals. The acidified isopropanol will convert the phenol red in culture medium to yellow-colored form that will not cause any interference while recording the absorbance of the final solubilized formazan product. So go ahead and perform the MTT assay.

I hope I have helped you.

All The Best!

Dear Amera

You can maintain the medium control in your 96 well plate and you can remove interference associated with the culture medium.

Hello Amera,

you have to use appropriate medium for your HIT_T15 cells without phenol red. Other mentioned components do not play crucial role in case of MTT test.

Best regards, Pavlina