What are examples of data-intensive research leading to totally new insights?

Data-intensive research is still all the rage these days. Breakthroughs in AI (and especially NLP) seem likely to only accelerate its adoption. But can anybody provide examples (preferably from...

17 January 2021 10,008 4 View

Can somebody recommend a dual expression vector?

09 January 2021 2,679 2 View

How to merge two Bold runs (rsfmri)?

Dear all, I have 2 Bold runs of a resting state MRI of the same subject. I want to combine these two into a single bold run. I habe tried so far to use fslmerge by time, but it seems not to be...

23 October 2020 4,480 1 View

How to estimate sample size for mixed ANOVA with several factors?

I would like to estimate the sample size for a 3b x 2b x 2w x 3w x 6w ANOVA [b=between subject factor; w=within subject factor]. I do not know much about the estimated effect size or...

16 July 2020 9,457 3 View

Where can I find information to justify the crosslinking of an antibody?

Hi all, I recently did a project where I did carboxyl-to-amine immobilisation with a monoclonal interferon gamma antibody (human ELISA development kit, Mabtech). I had carboxyl groups on a carbon...

30 May 2019 6,335 7 View

Are there any studies on the effects of the duration of short rests (breaks) in work on VDU?

need some valid results on the effects of short breaks while working with the computer and display.

20 June 2018 809 4 View

Is there a Software to depict multi-channel linguistic data transcripts?

Related to my PhD-research I am looking for software that is capable of displaying multi-channel transcripts of linguistic data. More precisely, I do have video data, that includes writte (chat)...

25 February 2018 5,263 2 View

Reverse transcriptase with 5'3' exo activity

Hi! I am looking for a reverse transcriptase that has no strand displacement but rather 5' to 3' exonuclease activity. The closest thing I found is Bst polymerase from NEB but that is only a weak...

24 July 2017 6,312 2 View

Is it allowed to conduct an ANOVA with a within-factor that has no variability?

Dear folks, does anyone know whether it is allowed to calculate a repeated measures ANOVA where one factor has no variability? E.g. I have 3 types of errors (factor categories: A,B,C) and 6 action...

24 July 2017 662 3 View

How to deal with strange AIC and BIC values in a latent class analysis?

19 June 2017 9,674 3 View

How two melting peaks in DSC of polypropylene can be merged into one crystallization peak?

The figure shows DSC cooling (blue) and 2nd heating (green) curves (step: 10 K/min) for a commercial sample supplied as Atactic Polypropylene (APP). The heating curve illustrates two major...

03 March 2021 5,857 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Necessary tube lenght to cool Natural gas in a dry-ice cooling bath?

I have a tank which contains natural gas at 250 Bar and 293 Kelvin. I want to cool the gas in a dry-ice cooling bath to about 195 Kelvin. The gas will flow in a copper-tube through the bath with a...

27 February 2021 8,128 1 View

Exit velocity of natural gas from pressurized tank?

Hi Guys. I'm a mechanical engineer and have a bit trouble, would be very glad if you could help me with my questions. I have a pressurized tank which contains Natural Gas at 200 Bar and 293...

26 February 2021 6,253 3 View

How can I verify my centrifugal fan design for electric motor cooling?

I have radial blade centrifugal fan design which will be mounted on exhaust side of electric traction motor. The fan will be mounted on same motor shaft inside the housing. How can I verify that...

23 February 2021 2,390 3 View

QPCR reaction efficiency with odd results what does this indicate?

I am working on a CFX 96 machine to determine reaction efficiency of my gene of interest. After performing a serial dilution of my cDNA template, I was able to find great reaction efficiencies for...

23 February 2021 809 3 View

Any interests in participating to an experiment in space?

Hello, The UN Office of Outer Space Affairs has a program called access to space for all that enables institutions to have hands on expertise related to access to space. There are three elements...

22 February 2021 7,980 3 View

Thermal and mechanical properties of concrete and steel after cooling?

How do the thermal conductivity, specific heat capacity, plastic strain, critical compressive displacement, and thermal expansion of both hot-rolled steel and concrete change after cooling based...

15 February 2021 1,385 5 View

Dealing with BMDM seeding and RNA extraction ?

biomedical master student here , i am dealing with mice BMDM and would like some help , i seed 0.5 x 10^6 cells/per well in a 6 well plate but when i extract the RNA i get a very low yeild , i...

15 February 2021 1,102 5 View