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I am bead-beating coral tissue (containing intracellular algae, bacteria, the coral animal, and a brittle coral skeleton) for up to 5 minutes (with 1-minute cooling breaks every 40 seconds) in...
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I'm attempting to extract RNA from coral using the trizol method + bead beating. To keep my samples from getting hot from bead beating I want to cool them every 20 seconds or so. I could cool...
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Milliq water isn't necessarily free of contaminating DNA molecules (especially from bacteria) even after autoclaving. Without purchasing special water that is certified DNA/RNA-free, how would...
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