Stephan Bitterwolf

5 Questions 4 Answers 0 Followers

Questions related from Stephan Bitterwolf

Can Calcium Carbonate impact Trizol Extraction Process?

I am bead-beating coral tissue (containing intracellular algae, bacteria, the coral animal, and a brittle coral skeleton) for up to 5 minutes (with 1-minute cooling breaks every 40 seconds) in...

07 April 2022 6,834 1 View

Trizol with Freeze Thaw Cycles?

I'm attempting to extract RNA from coral using the trizol method + bead beating. To keep my samples from getting hot from bead beating I want to cool them every 20 seconds or so. I could cool...

31 March 2022 7,983 2 View

How to make DNA (bacterial template)-free H2O?

Milliq water isn't necessarily free of contaminating DNA molecules (especially from bacteria) even after autoclaving. Without purchasing special water that is certified DNA/RNA-free, how would...

17 March 2022 6,960 4 View

Can I dissolve Estrone in DMSO?

I would like to use DMSO as a carrier for Atrazine and Estrone. I know it can be used for Atrazine. However, most people use ethanol for Estrone. Can I use DMSO as a carrier for Estrone? If so,...

25 June 2021 2,551 0 View

What are rapid method to homogenize pseudoparenchymatous red algal tissue?

Hi all, Has anyone ever successfully homogenized algal tissue with a bead beater? I am interested in phycobilin concentrations in the pseudoparenchymatous tissue of Mazzaella splendens. I've...

19 May 2016 2,485 1 View