Protocol for iNOS detection by flow cytometry?

I will perform an immunoassay to detect the production of iNOS in macrophages by flow cytometry. I need to know what concentration of surfactant I should use to permeate the plasma membrane. Does...

07 August 2018 5,407 4 View

Antitumor therapies classification?

Hello! How can we classify antitumor therapies that targeting non-cancer cells (like macrophages and endothelial cells) from the tumor microenvironment ?

11 December 2017 4,122 0 View

How to deplete circulating monocytes in mice?

Hi, I work with a drug whose antitumor mechanism of action probably involves the activation of macrophages in the tumor microenvironment. To test this hypothesis, I need to deplete the...

11 December 2017 1,365 4 View

McIlvaine buffer

A buffer is composed of a weak acid and the corresponding conjugate base, so what is the meaning of a phosphate-citric acid buffer (McIlvaine buffer). In what situations is it used?

07 August 2017 645 1 View

Reduction of cell numbers in the G2/M

06 July 2017 6,947 3 View

Can the HEPES buffer interfere?

Can the HEPES buffer interfere with experiments like Griess, ELISA and Zymography? Why?Thanks!

01 February 2017 6,038 0 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know to clarify these changes?

01 March 2021 1,290 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

How to calculate the crystallinity of cellulose film?

27 February 2021 2,753 4 View

What could be the reason for not having further low chi square value (better agreement) in my refinement result?

I have tried every combination of parameter changes and after that i have a poor chi square value. I can't get it lower any further. I know there have been a lot of discussion about Rietveld...

26 February 2021 8,480 3 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View