Can the HEPES buffer interfere with experiments like Griess, ELISA and Zymography? Why?
Thanks!
I would like to permeabilize cell membrane of macrophages (RAW264.7) to stain iNOS with anti-iNOS conjugated to Alexa Fluor 488. What concentration of Triton X-100 should I use? Does anyone know...
09 October 2018 6,045 3 View
I will perform an immunoassay to detect the production of iNOS in macrophages by flow cytometry. I need to know what concentration of surfactant I should use to permeate the plasma membrane. Does...
07 August 2018 5,556 4 View
Hello! How can we classify antitumor therapies that targeting non-cancer cells (like macrophages and endothelial cells) from the tumor microenvironment ?
11 December 2017 4,209 0 View
Hi, I work with a drug whose antitumor mechanism of action probably involves the activation of macrophages in the tumor microenvironment. To test this hypothesis, I need to deplete the...
11 December 2017 1,532 4 View
A buffer is composed of a weak acid and the corresponding conjugate base, so what is the meaning of a phosphate-citric acid buffer (McIlvaine buffer). In what situations is it used?
07 August 2017 901 1 View
Hello!My substance decreases the percentage of cell numbers in the G2/M phase (cell cycle) compared to the saline control in the cytometry assay. What can this suggest?
06 July 2017 7,063 3 View
Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?
07 August 2024 4,616 0 View
Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...
06 August 2024 5,373 2 View
Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...
05 August 2024 3,783 2 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View
Hello everyone! How can i prepare EDTA buffer solution (pH 8.2) to determine GSH levels? Thank you in advance.
29 July 2024 4,374 1 View
I want to preserve the sample leaves for the LM study of the stomata and conidia analysis. Whether 2.5% glutaraldehyde in 0.05 M phosphate buffer with pH 7 can be applied for preservation.
29 July 2024 8,560 0 View
// interested in the difference between floating events and short circuits.
22 July 2024 6,565 0 View
Our lab has extracted bacterial DNA samples using Qiagen DNeasy kit and eluted the samples in AE buffer. However, this buffer contains 0.5mM EDTA not suitable for downstream application. Any ideas...
13 July 2024 1,675 3 View
Commonly, we prepare 1.0% of agarose gel with 0.4g agarose powder and 40ml 1X TAE buffer. if I would like to prepare a 1.2%% of agarose gel, is it I need to add 0.48g agarose powder and 120ml 1X...
12 July 2024 2,058 5 View
I performed CV of Cu-MOF in a buffer solution of pH 8, and we did not find any peak corresponding to Cu2+ to Cu+. What is the reason behind this? Please give your valuable suggestions.
08 July 2024 2,388 4 View