Triton X-100 concentration to stain iNOS?

I would like to permeabilize cell membrane of macrophages (RAW264.7) to stain iNOS with anti-iNOS conjugated to Alexa Fluor 488. What concentration of Triton X-100 should I use? Does anyone know...

09 October 2018 5,951 3 View

Protocol for iNOS detection by flow cytometry?

I will perform an immunoassay to detect the production of iNOS in macrophages by flow cytometry. I need to know what concentration of surfactant I should use to permeate the plasma membrane. Does...

07 August 2018 5,407 4 View

Antitumor therapies classification?

Hello! How can we classify antitumor therapies that targeting non-cancer cells (like macrophages and endothelial cells) from the tumor microenvironment ?

11 December 2017 4,122 0 View

How to deplete circulating monocytes in mice?

Hi, I work with a drug whose antitumor mechanism of action probably involves the activation of macrophages in the tumor microenvironment. To test this hypothesis, I need to deplete the...

11 December 2017 1,365 4 View

Reduction of cell numbers in the G2/M

06 July 2017 6,947 3 View

Can the HEPES buffer interfere?

Can the HEPES buffer interfere with experiments like Griess, ELISA and Zymography? Why?Thanks!

01 February 2017 6,038 0 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

How to remove DCU and HOBT from my reaction?

I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??

28 February 2021 2,153 1 View