Tomas Machacek

5 Questions 17 Answers 0 Followers

Questions related from Tomas Machacek

Is DETA NONOate soluble in DMSO?

I wonder, whether DETA NONOate (NO donor) is soluble in DMSO. In the datasheet, solubility only in water or ethanol is explicitly declared. However, dissolving in anhyrous DMSO would be more...

11 November 2016 4,760 3 View

Which iNOS inhibitor should I choose to treat mice and ducks?

I would like to prevent iNOS activity in mice. Based on literature, I chose aminoguanidine as the inhibitor to be administered p.o. in drinking water. Which of these two compounds...

10 October 2015 5,947 2 View

Do you know any intravital fluorescent staining available for schistosome cercariae?

If you need to label living schistosome cercariae to track them intravitaly, what stain would you use?

04 April 2015 3,732 7 View

Is anybody experienced with this anti-iNOS antibody (Sigma) in Western blot assays?

Using the antibody linked in the end, I still get at least three non-specific bands in astrocyte lysates. Does anybody have the same problem? http://www.sigmaaldrich.com/catalog/product/sigma/n9657

02 February 2015 9,255 3 View

Does anyone have experience with the adding of 2-mercaptoethanol into media used for mixed glial cultures or microglia/astrocyte cultures?

I haven't used it and seen it in papers very often but my colleague uses it in case of macrophages. So I am just curious about your opinion/experience.

08 August 2014 5,848 2 View