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Questions related from Felipe Barros Teles
I would like to permeabilize cell membrane of macrophages (RAW264.7) to stain iNOS with anti-iNOS conjugated to Alexa Fluor 488. What concentration of Triton X-100 should I use? Does anyone know...
10 October 2018 6,008 3 View
I will perform an immunoassay to detect the production of iNOS in macrophages by flow cytometry. I need to know what concentration of surfactant I should use to permeate the plasma membrane. Does...
08 August 2018 5,516 4 View
Hi, I work with a drug whose antitumor mechanism of action probably involves the activation of macrophages in the tumor microenvironment. To test this hypothesis, I need to deplete the...
12 December 2017 1,452 4 View
Hello! How can we classify antitumor therapies that targeting non-cancer cells (like macrophages and endothelial cells) from the tumor microenvironment ?
12 December 2017 4,170 0 View
A buffer is composed of a weak acid and the corresponding conjugate base, so what is the meaning of a phosphate-citric acid buffer (McIlvaine buffer). In what situations is it used?
08 August 2017 847 1 View
Hello!My substance decreases the percentage of cell numbers in the G2/M phase (cell cycle) compared to the saline control in the cytometry assay. What can this suggest?
07 July 2017 7,024 3 View
Can the HEPES buffer interfere with experiments like Griess, ELISA and Zymography? Why?Thanks!
02 February 2017 6,121 0 View
