I am preparing 10mM Tris-HCl buffer for DNA aptamer with 200 mM NaCl, 25mM KCl, 10mM MgCl2 and a pH of 8. Because of the presence of MgCl2, the buffer is turning brown. Is it normal for the color to change or am i doing something wrong?
are you completely certain the salt used is magnesium (Mg) and not manganese (Mn) ? I do not know any coloured magnesium salts.
Paul is correct. Non of the constituent in the buffer is likely to give rise a "color".
I use MgSO4 and sometimes its brown. But color goes away after mixing the solution.
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