Transient Co-transfection of my gene construct having a GFP gene in it with the VSV-G envelope?

04 April 2013 0 4K Report

I want to do transfection in PG13 or in 293 gp cell line. I will be using Lipofectamine method. What should be the incubation temp, incubation time period and the amount of DNA for 10 cm petri plate?

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