5 Questions 17 Answers 0 Followers
Questions related from Saheem Ahmad
Please let me know the best way to produce monoclonal antibodies against any specific antigen. Please also let me know how much it costs for the production of monoclonal antibodies from...
05 May 2014 9,226 12 View
I want to isolate CRP from human serum. What is the best procedure to isolate it? With which compound does it bind most efficiently so that we can isolate it using affinity chromatography?
01 January 2014 384 1 View
I want to do transfection in PG13 or in 293 gp cell line. I will be using Lipofectamine method. What should be the incubation temp, incubation time period and the amount of DNA for 10 cm petri plate?
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My gene of interest is the T cell receptor gene (1383iTCR; 3 kb size) and I am trying to Ligate this gene into a pLVX-mCherry-N1 Vector (Lentiviral Vector; 8.7 Kb size). To match with the unique...
03 March 2013 2,213 5 View
How can I do the compensation? What measures should be taken into consideration while doing compensation of 5-8 different antibodies?
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