Saheem Ahmad

5 Questions 17 Answers 0 Followers

Questions related from Saheem Ahmad

What is the best and cheapest protocol for monoclonal antibody production?

Please let me know the best way to produce monoclonal antibodies against any specific antigen. Please also let me know how much it costs for the production of monoclonal antibodies from...

05 May 2014 9,224 12 View

Can anyone help me out for C-reactive protein isolation protocol?

I want to isolate CRP from human serum. What is the best procedure to isolate it? With which compound does it bind most efficiently so that we can isolate it using affinity chromatography?

01 January 2014 380 1 View

Transient Co-transfection of my gene construct having a GFP gene in it with the VSV-G envelope?

I want to do transfection in PG13 or in 293 gp cell line. I will be using Lipofectamine method. What should be the incubation temp, incubation time period and the amount of DNA for 10 cm petri plate?

04 April 2013 3,994 0 View

I am not getting the expected bands on an agarose gel after the digestion of the PCR products. I have performed PCR to add Mfe I and Age I Res. site onto the 5' and 3' ends of my gene.

My gene of interest is the T cell receptor gene (1383iTCR; 3 kb size) and I am trying to Ligate this gene into a pLVX-mCherry-N1 Vector (Lentiviral Vector; 8.7 Kb size). To match with the unique...

03 March 2013 2,211 5 View

Compensation of all wavelengths (colors) when analyzing for more than four antibodies in a single sample in a flow cytometry.

How can I do the compensation? What measures should be taken into consideration while doing compensation of 5-8 different antibodies?

02 February 2013 5,229 1 View