Data balancing before test/train split or only training data balancing. which is correct?

While reviewing technical articles, I encounter some papers where authors have used data balancing before splitting test and train (on full data), and some only balanced training data and let the...

04 February 2021 1,446 0 View

DFT Optical calculation and plotting of Real and imaginary Permittivity.??

Dear DFT Community, i have calculated Frequency dependent optical permittivity of Au using (VASP) my INCAR tag includes LOPTICS=.TURE., my OUTCAR has expected frequency dependent IMAGINARY...

30 December 2020 9,543 9 View

Where can I get historic global/national level hydrometeorological data (preferably gridded data)?

Hi, I would like to know where can I get historic global/national (preferably USA) level time-series data for the following (preferably gridded data)? Flood, Drought, Max temperature, Min...

08 November 2020 9,414 6 View

Which Stats to use with categorical IV and DV?

Can you guys tell me which stats should I use when I have both Dependent and Independent variable in categorical form I want to check level of Depression which is categorized as Normal, Mild,...

28 October 2020 3,173 3 View

Effect on MARK4 activity and microtubule of alzheimer mice brain?

I have to determine the effect of some phytoconstituent on MARK4 and microtubule of neuron. How can I do it. I mean do I have to first extract the MARK4 from hippocampus and cortex by RT PCR and...

12 October 2020 653 1 View

I need strains of Agrobacterium rhizogenes or Agrobacterium tumefaciens, how can I get them?

I need the above strains for my students .

02 October 2020 5,867 5 View

Can induce resistance may serve as a source to divert plant photosynthates to defense pathways?

Systemic acquired resistance (SAR) and induced systemic resistance (ISR) are two forms of induced resistance wherein plant defenses are preconditioned by prior infection or treatment that results...

21 September 2020 7,491 3 View

While inserting GOI in pCW57.1 lentiviral plasmid DNA, is it important to delete ccdB gene fragment?

I am facing a problem with the plasmid from addgene (pcW57.1), when transformed into chemically competent DHFa cells, did not give any single colony overnight in ampicilin containing agar...

17 July 2020 8,001 1 View

The reason of incomplete semi-circle?

The Figure in the attachment is taken from the following article: Preparation and characterization of a novel ionic conducting foam-type polymeric gel based on polymer PVdF-HFP and ionic liquid...

09 July 2020 8,409 4 View

Can anyone help to get zeta-potential from my Malvern zetasizer data files? I don't have software anymore?

I have my data files of Malvern zetasizer and I want to get zeta potential from that files anyone who can help me to read original files?

16 February 2020 8,141 5 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View