Shifa Javed

2 Questions 2 Answers 0 Followers

Questions related from Shifa Javed

Transfection_large plasmid_HEK cells?

Does anyone have any experience with transfecting 12Kb plasmid in HEK cell line using jet prime?

08 November 2019 1,528 3 View

Hi Everyone. i wanted to ask about extraction of proteins from cell lines for SDS PAGE and further western blotting?

I take the cells trypsinize the cells and wash them with cold PBS two times. Then i use RIPA lysis buffer according to the pellet and add protease inhibitors 1/20th volume of lysis buffer. Pipet...

17 March 2017 3,592 2 View