Try using these kits, they're optimized for both cell lines and have been used in siRNA delivery with around 70% transfection efficiency.

HCT116: https://altogen.com/product/hct-116-transfection-reagent-colon-carcinoma/

HT29: https://altogen.com/product/ht-29-transfection-reagent-colorectal-adenocarcinoma/

RNAiMAX works good for both.  

Hi Andrey,

Isn't the RNAiMax for RNA transfection? That's what it says on the thermofisher website

For HT29 cells, try using X-tremeGENE HP DNA Transfection reagent from ROCHE. It works really well!

DreamFect Gold works also really for these cells, you can check out this publication

https://www.ncbi.nlm.nih.gov/pubmed/28003121

if you want a sample you can ask us at [email protected]

Good Luck

Olivier

Try using these kits, they're optimized for both cell lines and have been used in siRNA delivery with around 70% transfection efficiency.

HCT116: https://altogen.com/product/hct-116-transfection-reagent-colon-carcinoma/

HT29: https://altogen.com/product/ht-29-transfection-reagent-colorectal-adenocarcinoma/