8 Questions 7 Answers 0 Followers
Questions related from Abraham Iwakun
Hi, I was wondering, would it be reliable to compare two cell lines using MTT if I plate them at different densities? For instance, cell line 1 at 1000 cells per well (because it grows faster),...
07 July 2019 529 3 View
Hi guys, So I am working with two colorectal cell lines (HCT116 and HT29). I extracted RNA, converted to cDNA and ran PCR for my gene of interest. Then also extracted protein and did a WB for the...
06 June 2018 5,370 5 View
Can anybody please explain how to do the selection step? I am currently growing my cells in 6 well plate and after puromycin treatment, I can see physical colonies (like the ones you see when you...
05 May 2018 6,970 1 View
HI guys, I have been trying to amplify NEIL3 gene in HCT116 and HT29 cells but no luck. I amplified GAPDH successfully but not NEIL3. Please has anyone had luck with NEIL3, if they can suggest...
10 October 2017 9,378 1 View
Hi guys,. I have done MTT assays with different drugs with 1000 or 2000 cells plated in 96 well plates. When I obtained my results, the line on the graph of cell viability against drug...
10 October 2017 8,889 5 View
Hi, Please does anyone have any successful experience with transfecting HT29 or HCT116 cells with DNA plasmid using Lipofectamine? I would appreciate any tips or advice. Regards. Abraham.
01 January 1970 2,638 6 View
Hi guys, I wanted to know, if I am conducting research experiments (such as transfection, MTT, etc) using 96 wells plates, would it still be ideal to generate my growth curve using 24 well plates?...
01 January 1970 2,793 2 View
I have done cell cultures in the past (during undergraduate and master's) where I used 10x PBS (for rinsing cells) directly from the manufacturer's bottle without any issues. Now that I am doing a...
01 January 1970 6,966 3 View
