Transfection of HT29 cells with siRNA and Lipofectamine 2000

More Anne-Marie Hewitt's questions See All

Non-parametric version of the wo-way repeated measures ANOVA?

Hi, can someone please help me with their expertise. To analyse a dataset with 2 IV´s (2 levels each) and 1 DV (time - measured at 4 points, i. e. 4 DV?) I found the two-way repeated measures...

24 July 2024 3,284 6 View

How to determine the acceptable amount of DMF when synthesizing MOFs?

I have synthesized MOFs already. However, I need to scale it up to produce 100g of MOFs. During my calculation, I need 15L of DMF just for this synthesis! So I thought, maybe DMF is just used as a...

01 July 2024 1,905 1 View

Should there be a difference in biotin management of Alopecia if the patient is a chronic energy drink user?

We have bumped into this energy drink, Celsius, with 1000% of RDA. I am starting to collect some information about this, but there is paucity of information.

08 June 2024 4,733 0 View

Is it normal to wash metal-organic frameworks with DI water for a week?

I have synthesized MOFs and been washing for about 2 weeks now until I reach the goal of UV absorbance of near 0.

27 May 2024 2,295 0 View

How can I identify an unknown DNA sequence & termination ?

Could you advise me on a simple technic for DNA sequence & termination definition (characterization) ? (I am non-biologist). Which parameters should I take in consideration ? Thank you

26 May 2024 1,590 3 View

How characterize double stranded DNA structure & conformation ?

I try to understand and identify my dsDNA structure. I need to know if my DNA has a special structure and it is double-stranded or not. Which method should I use ? Are FTIR and CD sufficient ? I...

26 May 2024 5,696 1 View

Does anyone have experience with AML/T cell cultures in serum-free medium?

We want to keep AML cell lines together with T cells in serum free medium for about 4-7 days. Currently we culture them in RPMI 1640 with 10% FCS, so something resembling that would be good I...

22 May 2024 1,763 0 View

Mediation possible with Response Surface Analysis?

Hello everyone, I'm doing my master's thesis right now and planning to use Response Surface Modeling. The plan is to test how the (in-)congruence of a personality trait of two people affects...

20 May 2024 9,811 0 View

How do you wash the modified biochar?

I usually wash it with MeOH by stirring (10 min.) then centrifuge at 8000rpm (10 min.) followed by DI water washing by stirring (10 min.) and then centrifuge again. I do it for 3x. The final one...

12 May 2024 8,835 0 View

How to look for Special Education MA or PhD Graduates that can validate a Questionnaire?

I am currently working on my Thesis proposal and I am looking for the best way to approach MA or PhD professionals in the field of Special Education. To be specific, I will determine level of...

06 May 2024 5,879 4 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View