Marwa Amer

5 Questions 12 Answers 0 Followers

Questions related from Marwa Amer

CPM assay with rotor gene Q?

Hi, I'm using the rotor gene q for my CPM assay and in the setup of the analysis I have to adjust the ramping rate to be 2˚C/min but the only way to do this is to adjust (wait for -- seconds for...

16 January 2017 9,482 0 View

Can any one help me with data analysis of Dual luciferase experiment?

Hi I did an experiment for miRNA and targets but I have a problem in calculating the significance of the data can you help me which test should I have to use and how to caluclte the p value or z...

29 June 2016 4,522 3 View

Does anyone have any advice about the best method for RNA isolation purity from paraffin blocks for RNAseq experiment?

Hi, I would like to ask about the best method to get high quality RNA to be used in RNAseq experiment from Paraffin blocks.

17 February 2015 9,945 1 View

Is there any common antibiotic that can work on both puromycin and neuomycin resistant genes for the selection of transfected cells?

I want to transfect Huh7 with two different vectors one containing neomycin resistance gene and the other containing puromycing resistance gene. Does one of them affect the other or is there any...

18 November 2012 1,271 9 View

Double Restriction digestion of a plasmid

I'm cutting miRNA expression vector with BamHI and NheI. I did the BamHI digestion by different conditions then cut with NheI. The problem is I have unexpected bands at some conditions and the...

11 September 2012 6,037 16 View