Marwa Amer

4 Questions 7 Answers 0 Followers

Questions related from Marwa Amer

CPM assay with rotor gene Q?

Hi, I'm using the rotor gene q for my CPM assay and in the setup of the analysis I have to adjust the ramping rate to be 2˚C/min but the only way to do this is to adjust (wait for -- seconds for...

16 January 2017 9,438 0 View

Can any one help me with data analysis of Dual luciferase experiment?

Hi I did an experiment for miRNA and targets but I have a problem in calculating the significance of the data can you help me which test should I have to use and how to caluclte the p value or z...

29 June 2016 4,494 3 View

Does anyone have any advice about the best method for RNA isolation purity from paraffin blocks for RNAseq experiment?

Hi, I would like to ask about the best method to get high quality RNA to be used in RNAseq experiment from Paraffin blocks.

17 February 2015 9,900 1 View

Double Restriction digestion of a plasmid

I'm cutting miRNA expression vector with BamHI and NheI. I did the BamHI digestion by different conditions then cut with NheI. The problem is I have unexpected bands at some conditions and the...

11 September 2012 5,991 16 View