Anne-Marie Hewitt

5 Questions 18 Answers 0 Followers

Questions related from Anne-Marie Hewitt

What would cause Cell Titre Glo assay wells to luminesce less than the media only wells?

Hi all, I'm doing a bunch of Cell Titre Glo assays to assess the effects of several inhibitors on 2 cell lines. Problem is, for some of the wells they seem to be luminescing less than the...

31 October 2020 1,757 2 View

Why do I get intermittent ladder smearing on just my front gel when I'm doing PAGE?

I've been doing some PAGE gels recently on PCR products, and every so often the front gel comes out smeared (including the ladder). I'm using the same reagents and procedure. It's not my technique...

02 December 2015 8,988 3 View

Can anyone recommend a good vector for stable expression in human colorectal cancer cell lines?

Anything that is fairly cheap, good expression levels and can produce stable transfections of cell lines such as HCT116 and COLO205.

03 December 2012 7,774 19 View

Transfection of HT29 cells with siRNA and Lipofectamine 2000

Can anyone recommend a protocol for transfection of HT29 cells with siRNA and Lipofectamine? Thanks for your input everyone, I seem to be having problems posting replies. I'll see if I can sort...

19 September 2012 8,904 2 View

Can anyone tell me why my RT-PCR reactions have a humped shape, followed by a gap then a "normal" reaction starting much later than it should?

I have used the same cDNAs and reagents at the same volumes as I have done on previous successful runs. The only difference is that it is a new probe. I put the housekeeping and gene of interest...

27 January 2012 7,286 21 View